FastQCFastQC Report
Wed 8 May 2024
EGAF00007545459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720074
Total Bases36.3 Mbp
Sequences flagged as poor quality0
Sequence length38-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC25590.35538014148545843No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT18490.2567791643636626No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC12210.1695659057263559No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC8900.12359840794140603No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA8830.12262628563175451No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTC7960.1105441940689429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)