FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750901
Sequences flagged as poor quality0
Sequence length40-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC26860.35770361206071105No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT19720.26261784176609165No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC16530.22013554383334155No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTC12550.16713255142821756No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA11600.15448108339181862No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT10430.1388998017048852No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT9260.12331852001795177No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT8170.10880262511303088No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC8150.10853627841752775No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT8030.10693819824450894No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG7980.10627233150575109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)