FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729
Sequences flagged as poor quality0
Sequence length45-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1692.5115173131223063No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT530.7876356070738595Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG520.7727745578837866Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC430.6390251151731312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA370.5498588200326943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC320.4755535740823302Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC290.4309704265121118Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG220.326943082181602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA200.2972209838014564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT140.20805468866101945Clontech SMART CDS Primer II A (96% over 26bp)
CCTTGATCTGGGGCCCTGAGGTCTCTGACTTGGCTTCCTTGGCCAGTCTGG140.20805468866101945No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC130.19319363947094664Clontech SMART CDS Primer II A (96% over 26bp)
ACCTAACTTCACATATCTGCAACCAGAGCAGCCACCAAGCATTACTTAGC120.17833259028087384No Hit
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT110.163471541090801Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC110.163471541090801Clontech SMART CDS Primer II A (96% over 26bp)
GCGAGGCCGAAGCCAAGAAGTGGATCGACAGCGAGTTCCAGCCATCGACCT100.1486104919007282No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG100.1486104919007282No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT100.1486104919007282No Hit
GTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGCCTATC90.13374944271065536No Hit
GGCCTCGCCGGGCCGATCCGGGCGGAAGACATTGTCAGGTGGGGAGTTTGG80.11888839352058254No Hit
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC80.11888839352058254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACGACGCTGGTATTGTCCTCTTCACAGATGCAGCCTACTATTCTCTTGTT80.11888839352058254No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT70.10402734433050972Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT70.10402734433050972Clontech Universal Primer Mix Long (96% over 25bp)
GCCGGCGCCGCCCATGTACTCGGTCAGCGACATGATCTTGCCGTAGCGGT70.10402734433050972No Hit
GTTCTGAGCTGCCCCTTGCCCACACACCCCAAACCTGTGTGCAGGATCCGC70.10402734433050972No Hit
GGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGATCTACGGATGA70.10402734433050972No Hit
TCTTCAGCTTCCTTCGACGCAAAACAGACTTTTTCATTGGAGGAGAAGA70.10402734433050972No Hit
CCTCCTCAGGTAGCTCAAATTGCTGCTAGGAAGATTTATAGGCAAAGAAGT70.10402734433050972No Hit
GCTTGTAATGGGCTCCACAGCGGGGGCATCGCTGGGCCTCGCCTTTGTGC70.10402734433050972No Hit
GCCGTCATAGATCGACTTGCCCGACTGCATCGAGGTCTGCAGTTTCTCGAC70.10402734433050972No Hit
GCTGCCTGGAGGGGATAGGGGGTCACACCCGTGGCCTGGTGGCCTCCCTGG70.10402734433050972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)