FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494
Sequences flagged as poor quality0
Sequence length41-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC260.7441327990841442Illumina PCR Primer Index 1 (95% over 24bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA170.48654836863194045RNA PCR Primer, Index 23 (96% over 30bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT150.42930738408700636Clontech SMART CDS Primer II A (96% over 26bp)
CCACTTGTTTCTAGTTTCCATTCATGCCACACATTAGACATGAGTTCTCCT100.28620492272467085No Hit
ACCCCTGAGCTGCGCAGTGCCATCCAACGACAGCAGTCCCATTTACTCAGC100.28620492272467085No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC90.25758443045220375RNA PCR Primer, Index 23 (95% over 24bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG90.25758443045220375Clontech SMART CDS Primer II A (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT90.25758443045220375RNA PCR Primer, Index 23 (95% over 23bp)
GTTCAGTGTTGGTGGAAATGAGCTGGTTTTTCAAGTCAAGACCCTGTCCCT80.22896393817973668No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC80.22896393817973668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC70.20034344590726963Clontech SMART CDS Primer II A (96% over 26bp)
GCTCGCTGGCTGCCCATGGTGCCCGGAAGAATGGGGGTGGCAGTGGCA70.20034344590726963No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC70.20034344590726963RNA PCR Primer, Index 23 (95% over 21bp)
CCCCTGAGCTGCGCAGTGCCATCCAACGACAGCAGTCCCATTTACTCAGCG70.20034344590726963No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT70.20034344590726963RNA PCR Primer, Index 23 (96% over 29bp)
ACATCACCTTATTATCATTTCATAAGTGCTCAAAATCTCACCACTTGTTTC70.20034344590726963No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG60.17172295363480253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGCCGTCTTCTGC60.17172295363480253RNA PCR Primer, Index 23 (95% over 23bp)
AGTCTGGAGAAGGGAACTTAGGTAGCAAGACTCGGTGGTGAAGTGAAGTTG60.17172295363480253No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT60.17172295363480253No Hit
CTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTA60.17172295363480253No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGCCGTCTT60.17172295363480253No Hit
CCCTACATCACCTTATTATCATTTCATAAGTGCTCAAAATCTCACCACTTG60.17172295363480253No Hit
GGCCTTCCCGGGCTCGGCAGGGACAGCGCTCCAGTACCCGCCTCCTGCCT50.14310246136233543No Hit
GTTAAGGCTTAGGGTATGATGAAATGGGACAGAAGAGTTAGTCTGGAGA50.14310246136233543No Hit
GCAGCCAGCCCCCGGCCTCCCCCAGCACACAGGAAGCCATTCAGGGAATGC50.14310246136233543No Hit
CCTACAGCTGAACTGTGGGAGTGCCACAGCTAGAGGGGGTGGGAAGAAG50.14310246136233543No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC50.14310246136233543Clontech SMART CDS Primer II A (96% over 26bp)
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCC50.14310246136233543No Hit
GCATCTGCCATGGCTTCCACAAAGTTGGCATCTTCTACAGTGTCACCAGC50.14310246136233543No Hit
GACTGGAGAAGGGAGGCGGCGGGCGAAGCGCACGTCGAGCGGGGGAGCGGC50.14310246136233543No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAA50.14310246136233543No Hit
CCTGTGTGCTGGGGGAGGCCGGGGGCTGGCTGCTGGGGTTGTACTCCAGCG50.14310246136233543No Hit
GATAGAAAAGAGGTTGTTAAAATTACACACTGTCCTACACTGCTGACC50.14310246136233543No Hit
CCCAGACAGGAGCCTGCTGGCTAGCATGCAAGCACTCCCCAAATTCTCTC50.14310246136233543No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA50.14310246136233543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAAACAGATACTGCTGAAATCTCAACTTCACTTCACCACCGAGTCTTGC50.14310246136233543No Hit
ACCCTGCCAGGCTCTGCAAAAGCATTGTCCCAGAGAACAGTGGCCGTCGC50.14310246136233543No Hit
GCTGCAGGCCAGTGAGGAAGTTGTCCCAGCAGGAGGAGCAGAAAAGCA50.14310246136233543No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC50.14310246136233543Clontech SMART CDS Primer II A (96% over 26bp)
GAGAAATCAAAGGGGCTCTCGCAAAGTTCTGGCTAAGAAGGAGAGATTGG50.14310246136233543No Hit
TCCAGGGCCTGGAGAGCCGCACCCAGCAGCCTTCGCTAACCCCTCTGTCT50.14310246136233543No Hit
CCAGGTTGGCAGGGGACCCTCTTCTCCCGTCTGCCCTGTGGGTTGCCCGCC50.14310246136233543No Hit
CGAGACCTCTCATTAGGTAAATTACATGGGAGTCAGACGCCCTTCGGCAGC50.14310246136233543No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA50.14310246136233543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT50.14310246136233543Clontech SMART CDS Primer II A (96% over 26bp)
CACTAGCCAGGAGATTCCTAGAAACTCTGATAAGAGTGTAACAGTG50.14310246136233543No Hit
GCAGACAACTGTGATGTCAGCCAATGTCACTCGTTCGCCCACCAGAAAAGT50.14310246136233543No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT50.14310246136233543No Hit
GCACAGAGTAACCAGCAAGGCCCGAGACCTCTCATTAGGTAAATTACATGG50.14310246136233543No Hit
GGTGGTGCAGTGGGTGAGCTTTGCTGATTCCGATATAGTGCCCCCAGCC50.14310246136233543No Hit
GGTATTGAGGGCCTTCAGGTCCTGGGAGCCCGACTGAGTGGCAAAGGCCTT50.14310246136233543No Hit
CCTAATAATGTGCCTTTGGTGAATTTACCCAAATTAGATGGTCCAGAAAA40.11448196908986834No Hit
CCTGAACTCTGAGAAGATGTCAAAATCCACAGGCAACTTCCTCACTTTGAC40.11448196908986834No Hit
AGTCCAGCTGGGTTGGCTGACGTCATCTCTGAGGGCTCTGCCAGGTGGATT40.11448196908986834No Hit
ACAGAGTCCCAGCCTCCTCTCCAAGAGGAAAAAAGTCAAGATGAAGCGGG40.11448196908986834No Hit
ACTATGTGAGCAATGAGGAGCTGCGGGGAAGTACTCCAGAGGCAGCAGCCC40.11448196908986834No Hit
CCAGTACCCGCCTCCTGCCTACCCTGCCGCCAAGGGTGGCTTCCAGGTTCC40.11448196908986834No Hit
GCCCAGGACTGGGTCTTGCTAAAGGTCTGTGGTCACTATTGCCTGGCTAA40.11448196908986834No Hit
GCACACAGGAAGCCATTCAGGGAATGCTGTCCATGGCCAACCTGCAGGCCT40.11448196908986834No Hit
GCTGCTGGTGGAGATGGATGGTTTTAATACAACAACAAATGTCGTCATTTT40.11448196908986834No Hit
GCAGGAGGAGCAGAAAAGCAAGAAAAAAAAGAGTGCCAAGAGGAAGCTGAC40.11448196908986834No Hit
CCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGG40.11448196908986834No Hit
GGTTGGGGTACTTGCGCATGCGGCTGGGTTTGATGAGCTGGGACTGGTAGC40.11448196908986834No Hit
ACTCTACCCAGGTGTACAGACGGAGAATACCTGCATCTGCCATGGCTTCC40.11448196908986834No Hit
GGCGGAAAGAAGGCTCTAGAACCTGCTTATAGAGCCACAACAGGGTGC40.11448196908986834No Hit
GTTCTGAGTCGGTTGTTCGACGCCCGGGGAAGGCCCCCGAGAGGGTCGTTC40.11448196908986834No Hit
GGAAGAATGGGGGTGGCAGTGGCAAGAGTGCAGGCAAACGACTGCTGA40.11448196908986834No Hit
CTCTGCTTTGTGTATAAATTAATGATGCTCTGTAGACTACCTAAGTTAATC40.11448196908986834No Hit
CCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGG40.11448196908986834No Hit
CCGACTCCTGCCTGCAGACCACGTGGGGAGCTGGCCAGGCCAAGGGGAGCT40.11448196908986834No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGCCGTCTTCTGCTTGA40.11448196908986834Illumina PCR Primer Index 5 (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT40.11448196908986834Clontech Universal Primer Mix Long (96% over 25bp)
CCTGGAAAGCACCATGTGAGACGCCTTGTGGCACTCAAGAAGAATGAAGTT40.11448196908986834No Hit
GCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACTGGGCAGAAATC40.11448196908986834No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC40.11448196908986834No Hit
GAGTACATGGGGAATTTCTCCGCAAATTTCCTGCCGGCAAGGTCCCAGCAT40.11448196908986834No Hit
CCTTTGTGATGCCTTAGGACATGTTTGAACTGCAGCAAAAAACAAAAACA40.11448196908986834No Hit
CTTCTTACCCAGGCCTGCACGCCTCATCCTGCCAGAGGAGAGAGAGAAA40.11448196908986834No Hit
CCTCTAAGCTAGTTAAGGCTTAGGGTATGATGAAATGGGACAGAAGAGTT40.11448196908986834No Hit
GCCGCAATGCCAGAGTCAAGAAGGAGAGTGGGAGCTCGGCAGCTGGCATCT40.11448196908986834No Hit
CGCAGAGTCGGGTGTCAGAGCCCAGTGGTTGAACAAAGCGTTCACTAGCC40.11448196908986834No Hit
ACTGCGGAGAAGCACTTGGCCCACTGCGCAGTAGCACTTGGCCCACTGCGC40.11448196908986834No Hit
GCCAGGGCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG40.11448196908986834No Hit
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT40.11448196908986834Clontech SMART CDS Primer II A (96% over 26bp)
CTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATT40.11448196908986834No Hit
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC40.11448196908986834Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG40.11448196908986834Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACATACCCGGGGTCTTGAAAATAATTCCATGCATGTGGGACTTTCAGA40.11448196908986834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)