FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325
Sequences flagged as poor quality0
Sequence length46-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT1416.064516129032258Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG1154.946236559139785Clontech SMART CDS Primer II A (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1014.344086021505376No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC903.870967741935484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA783.354838709677419Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC713.053763440860215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC562.4086021505376345Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA381.6344086021505377Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT261.118279569892473Clontech SMART CDS Primer II A (96% over 26bp)
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC220.946236559139785Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG220.946236559139785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC130.5591397849462365Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC130.5591397849462365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT120.5161290322580645Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC120.5161290322580645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGC110.4731182795698925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAAC100.43010752688172044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT100.43010752688172044Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC90.3870967741935484Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG80.34408602150537637Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT70.3010752688172043Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTC60.25806451612903225Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT60.25806451612903225Clontech SMART CDS Primer II A (96% over 26bp)
ACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC60.25806451612903225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC60.25806451612903225Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG40.17204301075268819Clontech SMART CDS Primer II A (96% over 26bp)
AGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCA40.17204301075268819Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGCTCTGTCCTGAGCTCATCCCCGTCTTCCGCCACCGCTCCCTTTTTCC40.17204301075268819No Hit
TACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACG40.17204301075268819Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACT40.17204301075268819Clontech SMART CDS Primer II A (96% over 26bp)
ACTAATGTAGAGAATGAATTGAATTGTGCAATGTTGCTGTTCTGGGGACTG40.17204301075268819No Hit
GAGTACATGGAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC40.17204301075268819Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTT30.12903225806451613Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.12903225806451613Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATG30.12903225806451613Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTAC30.12903225806451613Clontech SMART CDS Primer II A (96% over 26bp)
CCATGAACGGCAGGCACAACCATGGCTGCGTGATGACTCCGCAGAGCTGCG30.12903225806451613No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC30.12903225806451613Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC30.12903225806451613No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG30.12903225806451613No Hit
GGCCGCATAGCGGGCCGGATCGAACACCACCACGTCCGCGTAGTAGCCCGG30.12903225806451613No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCAAGT30.12903225806451613Clontech SMART CDS Primer II A (96% over 26bp)
GCAGGTCACCCGAGGACACGATGGGCTGGCCGTCGACCTTGCGGATCACGT30.12903225806451613No Hit
GCTACTACCACGGCTGCTTCGTTTGGACAAAAATAACCAGGAGGCATCCAC30.12903225806451613No Hit
CCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGTCAGTTAAAGAGGACT30.12903225806451613No Hit
CCCGGAGTTCCTGACCATGATGGCCAGAAAGATGAAGGACACAGACAGTG30.12903225806451613No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC30.12903225806451613Clontech SMART CDS Primer II A (96% over 26bp)
CATAATTGACCTGGCCATCTCCATCGATGTCAGCCTCCCTGATCATCTCAT30.12903225806451613No Hit
CCACCACAGCGCTGGCTGGGTGTGACGATGGGGTAGCGCACACTGCCATC30.12903225806451613No Hit
GCTCAAGGGATCACTACTAAGAATGGTTCCTCTTTGGGTAGAGCAGAGGG30.12903225806451613No Hit
AGCGTCAGATGTGTATAAGAGACAGGTATCAACGCAGAGTACATGAAGC30.12903225806451613No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT30.12903225806451613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)