FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097
Sequences flagged as poor quality0
Sequence length47-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT1155.484024797329519Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG964.57796852646638Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC833.9580352885073915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG713.385789222699094No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA602.8612303290414878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC562.670481640438722Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC552.6227944682880304Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA331.5736766809728182Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG291.3829279923700524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT261.239866475917978Clontech SMART CDS Primer II A (96% over 26bp)
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC251.1921793037672868Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC221.0491177873152122Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT140.6676204101096805Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC130.619933237958989Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG80.3814973772055317Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC70.33381020505484027Clontech SMART CDS Primer II A (96% over 26bp)
CCATGAACGGCAGGCACAACCATGGCTGCGTGATGACTCCGCAGAGCTGCG70.33381020505484027No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT70.33381020505484027Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTC60.28612303290414876Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG60.28612303290414876Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT60.28612303290414876Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC60.28612303290414876Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG50.2384358607534573Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC50.2384358607534573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATAC50.2384358607534573Clontech SMART CDS Primer II A (96% over 26bp)
ACTAATGTAGAGAATGAATTGAATTGTGCAATGTTGCTGTTCTGGGGACTG50.2384358607534573No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT50.2384358607534573Clontech SMART CDS Primer II A (96% over 26bp)
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT50.2384358607534573No Hit
GTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAAC40.19074868860276584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTT40.19074868860276584Clontech SMART CDS Primer II A (96% over 26bp)
CCACCAAGGAGTCGAGTGAGAAGGATCGCGGCCGGGACAAAACCCGAA40.19074868860276584No Hit
CTACATTCAAGTAATCCTCCTACTTCAGCATCCTGACAAGCTGGGCCTAC40.19074868860276584No Hit
ATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGC40.19074868860276584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGT40.19074868860276584Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG40.19074868860276584Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTGGAACCAGCTGTCAGCTCTGTAGTGAATTCCATCCAGGTTCTC40.19074868860276584No Hit
CATGGAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC40.19074868860276584Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCG40.19074868860276584Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30.14306151645207438Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGGAACTTAGGAGGGAAGGTGAGTTACAAGCTATACAGTTGCTTGGC30.14306151645207438No Hit
GAGACATGGATGGCTTCCCCGGGGTCCGGAACTATGGTGTGGTGGACCCGG30.14306151645207438No Hit
GTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTAC30.14306151645207438Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG30.14306151645207438No Hit
ACAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCTCCTCG30.14306151645207438No Hit
TGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT30.14306151645207438Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGG30.14306151645207438Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATCAGCGCCGCAGAGCTGCGTCACGTAATGACGAACCTGGGGGAGAAGC30.14306151645207438No Hit
GAGTACATGGAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC30.14306151645207438Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCC30.14306151645207438No Hit
ACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC30.14306151645207438Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.14306151645207438Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)