FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5740
Sequences flagged as poor quality0
Sequence length46-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA290.5052264808362369RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT150.2613240418118467RNA PCR Primer, Index 11 (95% over 21bp)
CCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGT150.2613240418118467No Hit
GCCGCAACAGGGCTCTGGCAAGCGGGGGACACCCGCGCTCTTACTTGTCG130.22648083623693382No Hit
GCTGGTCAGGAGGGATGCCTTCCTTGTCTTGGATCTTTGCCTTGACATTCT130.22648083623693382No Hit
AGAGATTGAGAGGCTTAAGACGAAGCAAAAAGAACTGGAGGCCAAGATGTT120.20905923344947736No Hit
GCAAAGAAACGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCACCC120.20905923344947736No Hit
CCCTAATACTGCACTTTTCCCATGGTCTTAGCAAACGGCATACCAGG120.20905923344947736No Hit
CCTGTCTGGGCCTCCCGAAGTGCTGGGATTACAGGTGCGAGCCACTGCACT120.20905923344947736No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC120.20905923344947736TruSeq Adapter, Index 11 (96% over 25bp)
CTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTGA110.1916376306620209Illumina PCR Primer Index 9 (96% over 26bp)
CCTGACCAGCAGAGGCTGATCTTTGCTGGAAAACAGCTGGAAGATGGGCGC100.17421602787456447No Hit
CCAAGTTAGTTAGCTTTTTTAAACTCACATCTCTTGATCAGTGTAGAGCG100.17421602787456447No Hit
TCTCAGAGGTGGGATGCAAATCTTCGTGAAGACACTCACTGGCAAGACCAT100.17421602787456447No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGC100.17421602787456447Illumina PCR Primer Index 9 (95% over 23bp)
CCCAGAGCAGCGGCTCTCCCCGCGGAGCGGATCAGTGCGGACCCTGCAGTG100.17421602787456447No Hit
CCTTACTTCTGGGCTCCAGGTGTAGCCTAAGCCCCCAGCTACTTTCACCTT100.17421602787456447No Hit
CCACTTCGAGGGTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGCC90.156794425087108No Hit
GCAAATAGTGAGCTAATAAATGGAGGTGGAGAAAAATAAAAGGCAAGGGAC90.156794425087108No Hit
GCAAGGAACCATCCATCACCACATTCCAGGGGTAAGCAAGTTGAGGTCTGT90.156794425087108No Hit
CTGAAAAAGGCTGTGGTGATGCCCCTACAGCTAATTCAGGAGCAGGCAGC90.156794425087108No Hit
CCAGGTGGCTGGTAGCCGCGGTTCACCAGCTTGGTCACCACTTTGAAG90.156794425087108No Hit
ATGGTATTGCTGGGTCTCTCAGGAATATGTATCATTTGATTTTGAGCATGT80.13937282229965156No Hit
GCCGCCGGCCGGCCACTGCAGGGTCCGCACTGATCCGCTCCGCGGGGAG80.13937282229965156No Hit
CCTCTGTCGCCAGGATGGAGTGCAGTGGCACGATCTCCACTCACTGCAACC80.13937282229965156No Hit
ACAATACTCCAGGGAGCTACGTCTGTGTGTGTCCTGACGGCTTCGAAGGA80.13937282229965156No Hit
GGTATACACTGGCTATTTAACCAAATGAGGAGGTTTAATCAACAGAAAAC80.13937282229965156No Hit
GCCCCCAGAGGTGGAGCCTACAGAGGCAGGCAGGCCTCCTTGAGCTGTGGT80.13937282229965156No Hit
CCTTGTCCTGGATCTTTGCTTTGACGTTCTCGATGGTGTCACTGGGCTCC70.12195121951219512No Hit
GATGTTGCGGGCCGTAGCGCGTGGGCGGCCGTGGGGTCTTGGGCGCTCGCG70.12195121951219512No Hit
TCTCCACGGCGGTAAAGACCAGAAGGCGGCGGAGAGCCACGCAAGAGA70.12195121951219512No Hit
GATAGATATCAGGGGAAAGGGATAAATCCCTGGAGACCCATCTCCCACCCT70.12195121951219512No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC70.12195121951219512No Hit
CTTAAGCTTGTAGAGGGTAGAAGCCAGGCTACACAGTGGAACATCCGCTCT70.12195121951219512No Hit
GTAATGTGCCGGACTTACCCTTTAAATTATTCAGAAGGATGTCCCGTGGA70.12195121951219512No Hit
CCAAAGTGTTTCATGTAAATAGAAAGCTGATTTTTCAGTCTCAGAAGTAGC70.12195121951219512No Hit
GCTCCCGCTAGGGTGCTGCAAATCTACTCCCGCCCGGAGACTCCGGAATAC70.12195121951219512No Hit
CGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGG70.12195121951219512No Hit
CCCCCTAGCAGCGGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCA70.12195121951219512No Hit
ACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGAC70.12195121951219512No Hit
GCTGGTCAGGAGGAATGCCTTCCTTGTCCTGGATCTTTGCTTTGACGTTCT70.12195121951219512No Hit
CCCATCTGTAGGAGGTGGGAACAGGACCCAGGCCAGTTGATCAGAGAGCTC70.12195121951219512No Hit
CCCTGAGAAGGCAAAAGAAAGAACCACTCATTGTCTTTGAGGAAGAAGATG60.10452961672473868No Hit
ACAGAGGCAGGCAGGCCTCCTTGAGCTGTGGTGGGCTCCACCGGGTTGG60.10452961672473868No Hit
CCTGCGTCTTAGGCTTCTCCACGGCGGTAAAGACCAGAAGGCGGCGGAG60.10452961672473868No Hit
GAGTACATGGGTGGGCGTGGTGGCTCACATCTGTGATGCCAGCACTTTGGG60.10452961672473868No Hit
ACTATCTATGTGGTTTTTATCCCATTCCAGGAAGCTGAACTAAAATTTTT60.10452961672473868No Hit
CCAAGAAATGCAGCTGTTTAAGGGACAAATGAGAATCAACCGTTAGAGAGC60.10452961672473868No Hit
CTGCTCCCCAGATGTTTTACAACTGGATTACAAAATAGGATCATCATTGAC60.10452961672473868No Hit
CCAAATGAGGAGGTTTAATCAACAGAAAACACAGAATTGATCATCACATTT60.10452961672473868No Hit
CTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGG60.10452961672473868No Hit
GCTTGAGAATCGGGTAGTACCCCTACTCGAATTGTAGTCTGGAGAGGCGTC60.10452961672473868No Hit
GCTTGGTCACCACTTTGAAGAGAAAGTGGGAGTAGTACTTGAAGGTGTTGT60.10452961672473868No Hit
CCCAAACTCCTGTCTTCTCGTCCCTTGGTCTATCTCCCTCTGGGCCCC60.10452961672473868No Hit
GCTGGAAGATGGGCGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC60.10452961672473868No Hit
GTGGTGATGCCCCTACAGCTAATTCAGGAGCAGGCAGCATCCCCAAATGCC60.10452961672473868No Hit
TTCTCTTCCGGCCTTTATTCTTCAGGATGTGGATCTCGGCATTTGGGGATG60.10452961672473868No Hit
GGGATAAACAGGTGTTTAAAAAAGAGAGGAGGTAAAAGGGAGGATGGGC60.10452961672473868No Hit
AGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGTAGTACC60.10452961672473868No Hit
GCCGCAGGTGGGTGAAGCGGAAACGTTTCAAACCGGGATGTTGCGGGCCGT60.10452961672473868No Hit
CCTTTATTCCGGGTTAGAACAAGGCCGTGCACACTGCAGACAGAAGAGCAC60.10452961672473868No Hit
GCTCACCAGGAGGGCAGAGAAGGGAGAGAATCCGTTTCTGTCACATGA60.10452961672473868No Hit
CCTCCCATCGTCCCTTAACAAAAGGATGATTTAATGATCTTGCTTGGGGTG60.10452961672473868No Hit
CATACTGTCTGGGATTAAGGGCAAATCTATTACTTTTGCAAACTGTCCTCT60.10452961672473868No Hit
GAGTACATGGGAGATTCCGAAAGTAGTGGCGAGCGAAATCGGAGAAGCCTG60.10452961672473868No Hit
GCTCTCTGGGGAACGTGTGTCCATTTGTAGGCAGTAGTCCGACACGTCGGG60.10452961672473868No Hit
CTGGCAAGCGGGGGACACCCGCGCTCTTACTTGTCGACTCGCGCGCGCTAC60.10452961672473868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)