FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5740
Sequences flagged as poor quality0
Sequence length45-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1091.8989547038327528No Hit
CCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGT190.3310104529616725No Hit
GCAAAGAAACGGGTGACAGATGGCACCTGGAAAATCGGGTCACTCCCACCC140.24390243902439024No Hit
ATGGTATTGCTGGGTCTCTCAGGAATATGTATCATTTGATTTTGAGCATGT130.22648083623693382No Hit
CCTGACCAGCAGAGGCTGATCTTTGCTGGAAAACAGCTGGAAGATGGGCGC120.20905923344947736No Hit
CCCCCTAGCAGCGGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCA120.20905923344947736No Hit
GCGGAGAGCCACGCAAGAGAAGAAGGACGTGCGCTCAGCTTCGCTCGCACC100.17421602787456447No Hit
GGTACAGGAGAGGATGGCAGGTGCAGAGCGGGCACTGAGCTCTGCAGGTG100.17421602787456447No Hit
CCACCTCGAGGGTGATGGTCTTACCAGTCAGGGTCTTCACGAAGATCTGC100.17421602787456447No Hit
CCTGACCAGCAGAGGTTGATCTTTGCCGGAAAGCAGCTGGAAGATGGGCGC90.156794425087108No Hit
GCCGCCGGCCGGCCACTGCAGGGTCCGCACTGATCCGCTCCGCGGGGAG90.156794425087108No Hit
CCCTAATACTGCACTTTTCCCATGGTCTTAGCAAACGGCATACCAGG90.156794425087108No Hit
GACTTCCGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTCTGC90.156794425087108No Hit
CCTTTATTCCGGGTTAGAACAAGGCCGTGCACACTGCAGACAGAAGAGCAC90.156794425087108No Hit
CCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGT80.13937282229965156No Hit
GTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCT80.13937282229965156No Hit
AGAGATTGAGAGGCTTAAGACGAAGCAAAAAGAACTGGAGGCCAAGATGTT80.13937282229965156No Hit
TCTCCACGGCGGTAAAGACCAGAAGGCGGCGGAGAGCCACGCAAGAGA80.13937282229965156No Hit
GCAAATAGTGAGCTAATAAATGGAGGTGGAGAAAAATAAAAGGCAAGGGAC80.13937282229965156No Hit
ACAATACTCCAGGGAGCTACGTCTGTGTGTGTCCTGACGGCTTCGAAGGA80.13937282229965156No Hit
GCTGGTCAGGAGGGATGCCTTCCTTGTCTTGGATCTTTGCCTTGACATTCT80.13937282229965156No Hit
GACTAAAATGGGAGGAGCAATGCGGTCTGTTCTTACTCAGGTTTTGACAA70.12195121951219512No Hit
CTGCTCCCCAGATGTTTTACAACTGGATTACAAAATAGGATCATCATTGAC70.12195121951219512No Hit
GCACAAGGCCGTCTTCAGGGGCCTGCTCACGGAGAAGGCGGCGCCCGTCAT70.12195121951219512No Hit
GCCATGGACAGCTTGGTTCCTGGGCCTGAGATCCGAGGAAGGCCAGCCCTG70.12195121951219512No Hit
CTCTGGGGAACGTGTGTCCATTTGTAGGCAGTAGTCCGACACGTCGGGGG70.12195121951219512No Hit
GCTGTTTAAGGGACAAATGAGAATCAACCGTTAGAGAGCAGGCAGCCTCTC70.12195121951219512No Hit
GCTTGAGAATCGGGTAGTACCCCTACTCGAATTGTAGTCTGGAGAGGCGTC70.12195121951219512No Hit
GCGCTCTTACTTGTCGACTCGCGCGCGCTACCCGGCTTTGAAAAGTCGCGG70.12195121951219512No Hit
GCAAGGAACCATCCATCACCACATTCCAGGGGTAAGCAAGTTGAGGTCTGT70.12195121951219512No Hit
GCACTTTGGGAGGCCAAGGCGGGCAGATCACGAGGTCAGGAGATCGAGACC70.12195121951219512No Hit
CGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGG70.12195121951219512No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCAT70.12195121951219512No Hit
CTCTAATGATATACAACTTACTCAGAATCTCATTACGGTCGTGTGCATGTT70.12195121951219512No Hit
CTGAAAAAGGCTGTGGTGATGCCCCTACAGCTAATTCAGGAGCAGGCAGC70.12195121951219512No Hit
CCCATCTGTAGGAGGTGGGAACAGGACCCAGGCCAGTTGATCAGAGAGCTC70.12195121951219512No Hit
CTGGCAAGCGGGGGACACCCGCGCTCTTACTTGTCGACTCGCGCGCGCTAC70.12195121951219512No Hit
GCCGCTGTCCACTGCAGGAGACGGCATCCTCAGGGCCACATTTTCCACGGG60.10452961672473868No Hit
GCAAAAGCACGCTTTTACGGTTTGGGCTATTCCCTTTTCGCTCGCCACTAC60.10452961672473868No Hit
CCAAGAAATGCAGCTGTTTAAGGGACAAATGAGAATCAACCGTTAGAGAGC60.10452961672473868No Hit
CCTAGCAGCGGAGGAGGGGACAAGTCGTCGGAGTCCGGGCGGCCAAGACCC60.10452961672473868No Hit
CCCCAGCGCACCGTGAAATGTCTGCTTGCTGCGCGGGCCGGTCTGTTTTC60.10452961672473868No Hit
CCATCCTGGCCGGGGAGGTCAGGGCCTGGGCGGCCAGGGCCCATTTCG60.10452961672473868No Hit
CACGGTACTAGTTCACTATCGGTCAATGATGAGTATTTAGCCTTGGAGGAT60.10452961672473868No Hit
GCCAGAGTGGGGGCACAAAATGCTGCAAAGAAACAGAAGCTGTCTTTGATC60.10452961672473868No Hit
GCTTTGAAAAGTCGCGGTCACTCACTGAGCGCTCTTCGGGCGCAGCGGTTC60.10452961672473868No Hit
GCTCCCGCTAGGGTGCTGCAAATCTACTCCCGCCCGGAGACTCCGGAATAC60.10452961672473868No Hit
GAAATAGGATGGGCCCTTCTTTATCACAGCTTGAGCTCAAAAAGGTTGGG60.10452961672473868No Hit
GCTCAGAGGGTCCTAAACCCATGGAGCCTCACTCATTGCTAGCACAGAAGT60.10452961672473868No Hit
GCCGGCGGCGGGTCTTGGCCGCCCGGACTCCGACGACTTGTCCCCTCCTCC60.10452961672473868No Hit
CCTTTTTCAGGGGCTTTGCCCCTGTGACTGTGCGATGTGAAAGGGGCCGG60.10452961672473868No Hit
ACTGAAGCCTAGGCAATGGTGGGCGTCCTTCCCCCAGTCTTGCTGCCACCT60.10452961672473868No Hit
GGGCGCGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAGGCCAAGGCGG60.10452961672473868No Hit
GCTGGTCAGGAGGAATGCCTTCCTTGTCCTGGATCTTTGCTTTGACGTTCT60.10452961672473868No Hit
GAGATTGAGAGGCTTAAGACGAAGCAAAAAGAACTGGAGGCCAAGATGTTG60.10452961672473868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)