FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253
Sequences flagged as poor quality0
Sequence length47-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1169.257781324820431No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG60.4788507581803671Clontech SMART CDS Primer II A (96% over 26bp)
GCAAGAAGACGGAAATGGCGCGGTTGTGCTGGATATCAGTAGTAGCAATGC60.4788507581803671No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT60.4788507581803671Clontech SMART CDS Primer II A (96% over 26bp)
ACTTGCTAGGAGGCGATGAGAATCTTAAGGTAAAAGCAAGCAGACCACATT40.3192338387869114No Hit
GGTATCAACGCAGAGTACATGGGGGAAAAGACATCAACCGAGATTCCGAA40.3192338387869114No Hit
GCACGAATGGCGTAACGACTTCTCTGCTGTCTCAACCACAGACTCGGCGA30.23942537909018355No Hit
GCACCAAGAGCCGATGAAGGACGTAGGAGCCTGCGATAAGCCCCGGGAAGT30.23942537909018355No Hit
GAGGTGCGCGTGTGCTACGACGTGACCAACGGCAACGTCTCGGTGGAGATG30.23942537909018355No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT30.23942537909018355No Hit
GTTACTGACACCACACCATGCTATAGTCACAAAATCACATATGCTTCCAGG30.23942537909018355No Hit
GTACATGGGAAGTTCCGACCTGCACGAATGGCGTAACGACTTCTCTGCTGT30.23942537909018355No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC30.23942537909018355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGAAGGAGGACCTCTACCTGAAGCCAATCCAGAGAACAATCTTGATG30.23942537909018355No Hit
GCTAGGAGGCGATGAGAATCTTAAGGTAAAAGCAAGCAGACCACATTAGTG30.23942537909018355No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTC30.23942537909018355No Hit
GATCAGGGTCCGAGGTTAGAAGTCCAATACGATCAGAGTGGTATTTCAAC30.23942537909018355No Hit
ACCGAGGTGCAGGCAGCGCGGGCGCCGCGCACCACAACGTGGCATGGATCG20.1596169193934557No Hit
CCGGCGGCAGCCGCTTGCTGCGGCGATGCACCACGTACCAGTTGAGCATC20.1596169193934557No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAG20.1596169193934557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGAAGATGCCGCCGCCGAGGCCGACCGCCTGCTCGACATGCTGAAGCGC20.1596169193934557No Hit
GCTCCAGATCGTCGACGATCTTCTCAAGCTGCTTCAACGCATCCTCGAAGC20.1596169193934557No Hit
AGGCACTAGAAGCCGATGAAGGACGTGGCAAACGACGAAACGCTTCGGGG20.1596169193934557No Hit
CCCAAGTAGCTGGGATTACAGGCATGCGCCACCATGCCTGGCTAATTTTTG20.1596169193934557No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC20.1596169193934557Clontech SMART CDS Primer II A (96% over 26bp)
GGTCGACTGCTGGCTGATGGGCAGCACGCCGTTCTTCTGCGCGCTCACGGC20.1596169193934557No Hit
GCCCAGCGCCAGCCCCAGCGCGCCCGTCAGCTCGAAGAGGCCGACCACGGT20.1596169193934557No Hit
GTATAAGATGGGGGGCGACATCGCCAACAGGGTACTTCGGTCCTTGGTGG20.1596169193934557No Hit
GGAGAGGTGAGGGTTAGGAACAACCAGAGGGGAAATACAAGAGGGTGACC20.1596169193934557No Hit
ACATCCAGGAGCGCCTGGCCGAGCTGAGCCAGAAGTTCAGCGAGAACGCGC20.1596169193934557No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT20.1596169193934557No Hit
TGTCCAGCCGGTGCACCGGGTACACATGTCGCCCCAATTGATCCCGCAGGG20.1596169193934557No Hit
CATGGACGCAGAACAGATCAACCAAATCGGCGCGATGCTCGCGGACCTG20.1596169193934557No Hit
CCGTTCTCGTCCCAGACGCGCTCGCGCGTGACGCGGCCCTTGGCGTCGA20.1596169193934557No Hit
CCTGAGTAGCTGGGACCGCAGGCGTGCCCCACACCCAGCTAATTTTTTGAT20.1596169193934557No Hit
GCACACCAGAGGTTCGTCCAACCCGGTCCTCTCGTACTAGGGTCAGATCCT20.1596169193934557No Hit
CCTTAAGTAAGCCTCAGCCAAAGGTTTTATGCATTGGACTTCCTGTGCCTG20.1596169193934557No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT20.1596169193934557No Hit
GCAGGGCTCGGTGAGCTTCGCACCCGCCGGCGCCGACAGCTGGTACGACGC20.1596169193934557No Hit
GGCACCCGGATGCAGCCTTTGTCGACCCTTCTGCCAATCTTCCAGAACCT20.1596169193934557No Hit
CGTTACGCGCGGCAGGACGAAAAGACCCCGGGACCTTCACTATAGCTTGGT20.1596169193934557No Hit
CCACACAGGTCATTTCACGTACGGGGCTATCACCCGCTATGGCCAGCCTTT20.1596169193934557No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC20.1596169193934557No Hit
CACTATAGCTTGGTATTGGTGTTCGGTTCGGTTTGTGTAGGATAGGTGGG20.1596169193934557No Hit
AGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTC20.1596169193934557No Hit
CCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTCTTCCTTCCGCTGAGCT20.1596169193934557No Hit
CCCTCTCAAACACCCTGAACTGGGTGTTGTTTTTTGTTTTTTTTTTGAGAC20.1596169193934557No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG20.1596169193934557No Hit
CCTTGCCCATGCCGGTGAGGATGATCCCGATGGCGTTCTTGCCCGCGTGCT20.1596169193934557No Hit
GTCGGTACTGAGATGTTTCACTTCCCGACGTTCCCTCCAACTACCCTATGT20.1596169193934557No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG20.1596169193934557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGATGCCGTCGGCTCGCAAGTGCAGCAGCCGGTCGGCCTGCGCAGCCGCG20.1596169193934557No Hit
GCGGCCCGCCGGCATCGGCAACCCGAAGGACTTCCGCAACGAGATCGTCA20.1596169193934557No Hit
GATCAGAGTGGTATTTCAACAACGACTCCATGAACACTGGCGTGCCCACTT20.1596169193934557No Hit
GGCTGGAAGGGGGGATCAACAGCGCCTTCCAGCGTCCCAGTAGCCTCCCCT20.1596169193934557No Hit
CTTTTCATCCAGGTAAATTTTCTTGACTTTATTTCTCTGCTTCTCATTT20.1596169193934557No Hit
CCAAAGCCACCAGATAAGCCGCTGATGCCCTACATGAGGTACAGCAGAA20.1596169193934557No Hit
GAGTACATGGGGGAAAAGACATCAACCGAGATTCCGAAAGTAGTGGCGAGC20.1596169193934557No Hit
GCAGACCACATTAGTGATGTGCATGTGCTCCTGGTGAGATTTAATAACAC20.1596169193934557No Hit
TCGGTGTTGACGTCGGAGTGCGCATCGAGCCACAGCACGCGAAGCTTCTTG20.1596169193934557No Hit
CGACGTGCTCGAGGTGTTCGGTCATGAGGCGCACGGCGCGCTCCTCGTCGC20.1596169193934557No Hit
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT20.1596169193934557Clontech SMART CDS Primer II A (96% over 26bp)
GACCGCTGCCGTCCATCACGAAGTGCGCGTGGCGCGTGGCCGCGCAGTTGG20.1596169193934557No Hit
GCTGATGTCGAGGTCGCACATGATCTTGACGCGCGCGACGATCGCGTTGCG20.1596169193934557No Hit
CCAATACACATTACGGAAGAGGTGTGCAGGCCATTCCTGGGTACAGAGA20.1596169193934557No Hit
GCGGGATAGGACGAAGGCGATGGGCATGGGGAGGTGGAGACGGAGGGCAGT20.1596169193934557No Hit
CCTGAAGCAGGGGGACAGGTGTACAGACATGTTCTTGTGGCGCTTCTCGA20.1596169193934557No Hit
CTTATTGTGAACTTCAATAGGACCAAGTTCAAAGGCAAATCACGATAAAA20.1596169193934557No Hit
GTATCAACGCAGAGTACGCGGGCCCGTCGCTCAGCAGGAAGTCCTTGAAGG20.1596169193934557No Hit
GCCGCCGCAGCGGCTGATGCTGGCGGCGGTGCCGCTCTACAGCATGGGCGT20.1596169193934557No Hit
GCTGTTTCCTGGGGGCGCTGGGAGGCTGCCACTGAACTTCACCCATCAGAC20.1596169193934557No Hit
CCCAAAGTGCTGGGATTACAGGCGTGAACCACTGCGCCCGGCCTCATTTGC20.1596169193934557No Hit
GCGCTCGAACCGCTGTTCACGAACTCCCGCTACACCATGGGCGTCGACATG20.1596169193934557No Hit
GCTATGGCCAGCGTTTCCAAGCTGTTCCACTATTCAATCGACTATCACGT20.1596169193934557No Hit
CATGGAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC20.1596169193934557Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCAGGAGCAGCCCCTCTGCGCTGGGCCTACTGCCCTTGGGGACCGAGATC20.1596169193934557No Hit
GGAGCTGAAGCTGAAGCTGTTGCCGCTAAGTCTAACGAAGAAGTAGGTTCT20.1596169193934557No Hit
CTGGGTTGATAGGCCGGATGTGGAAGCGCAGTAATGCGTGAAGCTGACCGG20.1596169193934557No Hit
CCTGGCACTCTTCCGACAGGGACTTCGGCAGCTTCGAGCACATTTTGTCA20.1596169193934557No Hit
GACTAGCGAAGGCTTTGAAATTCTTCTCTTCCTCAGTGATGACTCGAGCTT20.1596169193934557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)