FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090
Sequences flagged as poor quality0
Sequence length46-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC181.651376146788991RNA PCR Primer, Index 26 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA151.3761467889908259RNA PCR Primer, Index 26 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC141.2844036697247707RNA PCR Primer, Index 26 (100% over 23bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA80.7339449541284404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC80.7339449541284404No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG70.6422018348623854Clontech SMART CDS Primer II A (96% over 26bp)
GCAAGAAGACGGAAATGGCGCGGTTGTGCTGGATATCAGTAGTAGCAATGC70.6422018348623854No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGC60.5504587155963303TruSeq Adapter, Index 3 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT50.45871559633027525RNA PCR Primer, Index 26 (100% over 22bp)
ACTTGCTAGGAGGCGATGAGAATCTTAAGGTAAAAGCAAGCAGACCACATT40.3669724770642202No Hit
GCAGACCACATTAGTGATGTGCATGTGCTCCTGGTGAGATTTAATAACAC40.3669724770642202No Hit
CACGGTACTAGTTCACTATCGGTCAATGATGAGTATTTAGCCTTGGAGGAT40.3669724770642202No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT30.27522935779816515RNA PCR Primer, Index 26 (100% over 28bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTT30.27522935779816515RNA PCR Primer, Index 26 (96% over 28bp)
GCGGTTGTGCTGGATATCAGTAGTAGCAATGCTGTTGTTAACTTCGACGAT30.27522935779816515No Hit
GTCAGCTTCACGCATTACTGCGCTTCCACATCCGGCCTATCAACCCAGT30.27522935779816515No Hit
AGGCACTAGAAGCCGATGAAGGACGTGGCAAACGACGAAACGCTTCGGGG30.27522935779816515No Hit
GCATATACAACAGTATGCAGTTAATGAGCGACCCGAATATGTTGAAAGTAT30.27522935779816515No Hit
CTTCAGGCCAAGGCTTTCCATGTCAAGAAGCATCGAACAAGAACGAGACTG30.27522935779816515No Hit
CTGGCGGGGGCCCAGCATGGCTTCCTGGGGCGCTGCCTGGGTCCATTGC30.27522935779816515No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTC30.27522935779816515RNA PCR Primer, Index 26 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT30.27522935779816515RNA PCR Primer, Index 26 (95% over 22bp)
CCAGGCACTGTCCCTGAACCCGATCAGGGTCCGAGGTTAGAAGTCCAATAC30.27522935779816515No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT30.27522935779816515Clontech SMART CDS Primer II A (96% over 26bp)
GCACGAATGGCGTAACGACTTCTCTGCTGTCTCAACCACAGACTCGGCGA20.1834862385321101No Hit
GCATGACGGTGGCCGCCATGTGCGCTGCCACCATCCAGTACAGCGACAACG20.1834862385321101No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA20.1834862385321101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTACTAAGATGTTTCAGTTCTCCGGGTCTGCCTTCTGACATGCTCTGTC20.1834862385321101No Hit
ATGTTGTAGCCACAGCTGGAGCCTGAGTCCGCTGCACGGAGACTCTGGTGT20.1834862385321101No Hit
CCCAGGCTACTTTGGGAAAGTTGGTATGAAGCATTACCACTTAAAGAGGA20.1834862385321101No Hit
CCACCAATGCAGGCATGACAAGTGGCTCCCATATAGTCTCCAAGTGCC20.1834862385321101No Hit
GGTATTAGGAATATTATGAGTGACTTAATAGATTACTTTGGTTTAGTCAA20.1834862385321101No Hit
CCGGCTTCGCGCCGCGTGCGCGCATCGAGCTCAACTACGACGCGGCCATGC20.1834862385321101No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC20.1834862385321101Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGTTGGCAACCGAGCGTTGATCCGGGGATGTCCGAATGGGGAAACCC20.1834862385321101No Hit
CCACGTCACTGCGTCACTTTCATCGTATAGCAGGTGCGGGAATATTAACCC20.1834862385321101No Hit
GCTGAAGCTGAAGCTGTTGCCGCTAAGTCTAACGAAGAAGTAGGTTCTGG20.1834862385321101No Hit
CCTGTCGGTACTGAGATGTTTCACTTCCCGACGTTCCCTCCAACTACCCT20.1834862385321101No Hit
CCACATTAGTGATGTGCATGTGCTCCTGGTGAGATTTAATAACACAAAAT20.1834862385321101No Hit
GAGTACATGGGGTGGTACTGAGCTAACGACAAGTAGGGCGGGACACGAGA20.1834862385321101No Hit
GCTCTTCAGGAACTCAGCGTTTCTCTTAGACCTCGAGCCTCCGAGAAGACG20.1834862385321101No Hit
CAAGGGATGGGAGGGCTGTGGCCCATTAATCAAAAGCTGAGCATCGGGC20.1834862385321101No Hit
ATAATCACTGCGCCCGCTCATAAGGGGATGGCCATGGCTAGGTTTAT20.1834862385321101No Hit
GGGAAAAGACATCAACCGAGATTCCGAAAGTAGTGGCGAGCGAAATCGG20.1834862385321101No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC20.1834862385321101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAGAGAGGGAGGATATGATGGTGCTTCACTGGGAATGTGCTAAGAACATT20.1834862385321101No Hit
CCTCCATGCCCGGCTAATTTTTGTATTTTTTAGTAGAGACTAAATGTAGT20.1834862385321101No Hit
CTTAACTCTCCTCCCATTCTCGCCCCTTTCAACTTATCCCCACCCCGAAGT20.1834862385321101No Hit
GGCGATGGGCATGGGGAGGTGGAGACGGAGGGCAGTGAGAATGAGGGGGC20.1834862385321101No Hit
GTGACACACGCTGGAGCTGAAGCTGAAGCTGTTGCCGCTAAGTCTAACGA20.1834862385321101No Hit
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC20.1834862385321101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACAAGCAGGGCAAGGTGCGCGCCCTGGCCGTGACCAGCCGCGAGCGCA20.1834862385321101No Hit
CCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCACGC20.1834862385321101No Hit
GTCGGTACTGAGATGTTTCACTTCCCGACGTTCCCTCCAACTACCCTATGT20.1834862385321101No Hit
GTTATTGGGGGCAGAAGATGGCAAACATCCAGACTTCGGAGCCACTTTGT20.1834862385321101No Hit
GATTCTTGCTGGCAGATAACCTGTATTGCAAAGCTTCAGTTCCTCCTACCG20.1834862385321101No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG20.1834862385321101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTAGAAATCCCCCTTTCTCTAATAGCGAGGCTCTAACCACACAGCCTAC20.1834862385321101No Hit
AATATGAGACCAGGGAGATTAAGCATGCGGGATAGGACGAAGGCGATGGGC20.1834862385321101No Hit
CCTCAACTCCTGGCTCAATACTGATGGAAGTAATCTGCTTAACAATCTC20.1834862385321101No Hit
ATAACGGAACAGCTTGGAAAGGCTGGCCATAGCGGGTGATAGCCCCGTACG20.1834862385321101No Hit
CCATCGCCATCAGTCTTTCCAACCCGTGACGCAGGATGTCGGGCAGGTCGG20.1834862385321101No Hit
CTTCCGGGGGCGACACCTGCGGCCCGGCGGAGCCGGTTCCGCGGTGTCCCG20.1834862385321101No Hit
GAGTACATGGGACCTGGTGGTGATGACATGGGACCTTGCTGGTGAGGATAC20.1834862385321101No Hit
GGCCAGAATGACTTTGCTTGGAGTAGAGCCGTCGTGATTTGTCAGAATGA20.1834862385321101No Hit
CAGAGTAGGGAGCCTGTGGCTGGCAAGCTCTGCACCATTTTCCTATCTG20.1834862385321101No Hit
ACCCTGTGCCCCCTCATTCTCACTGCCCTCCGTCTCCACCTCCCCATGCCC20.1834862385321101No Hit
CACCTACTACCTGGACATCACCAACCCGCCGCGTACCAACGTGCTGTACG20.1834862385321101No Hit
CTATCCTTATCTGTCTGGTCGAGTCATTCTGGGCTGACTGGGCACCATCAT20.1834862385321101No Hit
GAGTACATGGGGAAAGCATCTAAGCGGGAAGCCGGCCTCAAGATGAGACTT20.1834862385321101No Hit
CCTGCCCTGGATGGTTCCCTGGGGGATGGAAGCTCTGGGTTCCTGGGGCC20.1834862385321101No Hit
AAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTTAGAAC20.1834862385321101No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTGA20.1834862385321101RNA PCR Primer, Index 26 (96% over 29bp)
GGAGGGGCAGGCTTGCGAGCTGCATTTGGCCTTTCTGAGGCAGGGTTTAAT20.1834862385321101No Hit
GCGTCTAGAGGAGGACTGGCAAAGCCAGAAGGCCAAATTGCAAGCCCAGGT20.1834862385321101No Hit
CCCAACTTTGAGGCCAATACCACCGTCGGCCGCATCCGTTTCCACGACTTT20.1834862385321101No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTG20.1834862385321101No Hit
GGTGGCGGAAGGTGATGCGGCCCTTGGTCAGGTCGTAGGGCGAGAGCTCG20.1834862385321101No Hit
GCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTC20.1834862385321101No Hit
GGCGGATCCGGGTGTGGCGGGGACGAGAATCGCTCCCATGCTTAACCCTG20.1834862385321101No Hit
CCCAAAATGCTGGGATTACAGGGGTGAGCCACCGTGCCCAACCTCACTTGC20.1834862385321101No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTC20.1834862385321101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)