FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090
Sequences flagged as poor quality0
Sequence length46-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG877.9816513761467895No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT100.9174311926605505Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA70.6422018348623854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGCTGAAGCTGAAGCTGTTGCCGCTAAGTCTAACGAAGAAGTAGGTTCT50.45871559633027525No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG40.3669724770642202Clontech SMART CDS Primer II A (96% over 26bp)
ACTTGCTAGGAGGCGATGAGAATCTTAAGGTAAAAGCAAGCAGACCACATT40.3669724770642202No Hit
GCTGTATATGTTAGCTTGTGTAAGAATGTGTTTTCAAAACAGTGTGCGTGG30.27522935779816515No Hit
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT30.27522935779816515Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC30.27522935779816515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTAGTTCACTATCGGTCAATGATGAGTATTTAGCCTTGGAGGATGGTCCC30.27522935779816515No Hit
CACTATCGGTCAATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCCATAT30.27522935779816515No Hit
GAGTACATGGGTGGCACCAAGAGCCGATGAAGGACGTAGGAGCCTGCGAT30.27522935779816515No Hit
CACGGTACTAGTTCACTATCGGTCAATGATGAGTATTTAGCCTTGGAGGAT30.27522935779816515No Hit
TCCATGTCTTCTTCAACGAGAGCTTCCGCTACGTGCGAGAGCTGAAGTCGG20.1834862385321101No Hit
GTATTAAAGGGTATGATGTGATTGCTCAAGCTCAGTCAGGTACTGGCA20.1834862385321101No Hit
CATATTAGGGGCTTCGGCTTTTACACTGAATTGGTTGAGAAGTCACTGGG20.1834862385321101No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA20.1834862385321101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATATGGGGGGCCCTCCCATGATGCCAATGATGGGCCCTCCTCCTCCTGGG20.1834862385321101No Hit
GGGAACTAGCCAGGACCTCTGACCAAGTCCCTTTCTGTTTAGAGTTGCC20.1834862385321101No Hit
GCCAACTTACAGATTTACTAGCCTTGATCCTAGAATTCATGGTGAAAAACC20.1834862385321101No Hit
GCCTTTTAGGTCATCGCATACTGAATACATAGGTATGCGAAGCGAACGCGG20.1834862385321101No Hit
TCGGGTGAGAGGCTCCCAGCCAGACTACTGGGTTGATAGGCCGGATGTGG20.1834862385321101No Hit
GGTGGAGACGGAGGGCAGTGAGAATGAGGGGGCACAGGGTAGCCCGGCTTG20.1834862385321101No Hit
GCTTCATCACCCCAGTTTCCAGAGGCACTAAATGAGATGGAGATAAAGA20.1834862385321101No Hit
CCAATCCACGAGTGGCAGCCGCGGGACTGTCGCGTCGGCGCCCGACGCCGG20.1834862385321101No Hit
CCTTGAACCTGCTTAAGAAAAAGCACCTAAGTTTTGGGTGGAGCTGTTTCT20.1834862385321101No Hit
ACATAAGAGGGGAGAGTCCAAAGAGAGACCTTAATCACTGCAAGACTCTCC20.1834862385321101No Hit
CACCACCAGGTCCCATATCCTCACCAGCAAGGTCTCATGTCCTCACCACC20.1834862385321101No Hit
GGGAAAAGACATCAACCGAGATTCCGAAAGTAGTGGCGAGCGAAATCGG20.1834862385321101No Hit
CCAGTTGTCCTCCTCCATGTTCCCCAGAGCAGCATTGATTCCTCCCTGTGC20.1834862385321101No Hit
GCGTAGGGGAGCATTGAAACCGGCGTTGAAGGTGTGGCGTGAGCCATGTTG20.1834862385321101No Hit
ACTTCTTCGTTAGACTTAGCGGCAACAGCTTCAGCTTCAGCTCCAGCGTGT20.1834862385321101No Hit
ACTTTTCTGTGGGCTCTTCACAATTGTAAGCATTGATATCCTTGCTCCAGG20.1834862385321101No Hit
CCAACTACCCTATGTGTTCAGGTAGAGGTAACTGGACATGACTCCAGCTGG20.1834862385321101No Hit
GCAAGAAGACGGAAATGGCGCGGTTGTGCTGGATATCAGTAGTAGCAATGC20.1834862385321101No Hit
CGTTACGCGCGGCAGGACGAAAAGACCCCGGGACCTTCACTATAGCTTGGT20.1834862385321101No Hit
CATTAGTGATGTGCATGTGCTCCTGGTGAGATTTAATAACACAAAATATG20.1834862385321101No Hit
GTGAAAGGACCAGTTCGAATGCCTACCAAGACTTTGAGAATCACTACA20.1834862385321101No Hit
GCTACCCTGTGCCCCCTCATTCTCACTGCCCTCCGTCTCCACCTCCCCATG20.1834862385321101No Hit
GGATGACAAAGAGGTGTTAGAAACTGATTTTTGGAAGCACGGCCTCTCTGC20.1834862385321101No Hit
GCACCCAGGGGCACTGGCATCGTCTCCGCACCTGTGCCTAAGAAGCTGCTC20.1834862385321101No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC20.1834862385321101Clontech SMART CDS Primer II A (96% over 26bp)
ACCCGGAGCAGCCATGGGTCCTCAAACAGGCCTCCACGGCCAGGTGGGCTC20.1834862385321101No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATC20.1834862385321101No Hit
ACGAAGAAGTAGGTTCTGGAAGATTGGCAGAAGGGTCGACAAAGGCTGCAT20.1834862385321101No Hit
GAGTACATGGGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGC20.1834862385321101No Hit
CCTAGTTCATATACAAAGTGGCTCCGAAGTCTGGATGTTTGCCATCTTCTG20.1834862385321101No Hit
ACCACTGCCGTCGTTCCTGTTGAAGCGAATCGCGCGTCCGTCTCGCGCTCC20.1834862385321101No Hit
GATCGCACCACATCAATGATGGGAGCAGCCCCCGTCTTGTTTTTAGCAGC20.1834862385321101No Hit
CCTTAAGCTGCACATCCAGGTCCTGAATCCTCTCCTTCAGCTTCTGGGCGT20.1834862385321101No Hit
GGGCAGTGAGAATGAGGGGGCACAGGGTAGCCCGGCTTGTGGCACCCGGAT20.1834862385321101No Hit
AGCATGTCAGAAGGCAGACCCGGAGAACTGAAACATCTTAGTACCCCTGTC20.1834862385321101No Hit
TATCAGTGTAAGGTTTGTCCGAAGGCCTTTGCACAGAGCTCCTCCCTCATT20.1834862385321101No Hit
GCCGCAGGGACGGGCAGTCCTGCAGGATGGCCAGGATGTGCCTCCGGGCCT20.1834862385321101No Hit
CGAAGAAGTAGGTTCTGGAAGATTGGCAGAAGGGTCGACAAAGGCTGCATC20.1834862385321101No Hit
GCGAGCAGCACGAAAGCGATGCACCACCATGCCACCTGCAGGTCGACG20.1834862385321101No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCAT20.1834862385321101No Hit
CACAGGGTAGCCCGGCTTGTGGCACCCGGATGCAGCCTTTGTCGACCCTTC20.1834862385321101No Hit
GGGATGCTGGTGTTGAGTTCGGGCTCGGTGCGGTCGAGCCTGAAGGTCTGG20.1834862385321101No Hit
ATGAAGGACGTGGCAAACGACGAAACGCTTCGGGGAGCTGTAAGCAAGC20.1834862385321101No Hit
GGCCATATTGACTTCTGTGGTAGTAAATTGATCTCGAAGAAAGTCAAGGTC20.1834862385321101No Hit
GCACTTTGGGAGGCCGAGGAGGGCAGATCACTTGAGGTCAGGGGTTCAAT20.1834862385321101No Hit
GTGTTATCAAGTCATCGGCTTATTAGTACCGGTCAGCTTCACGCATTACTG20.1834862385321101No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTG20.1834862385321101No Hit
TCTCCCAGCGCGGTGTTTCCGTCGCGAGGAAGCGCGCGAAATCCTCGCTGC20.1834862385321101No Hit
GCCATGGAATCGGGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGC20.1834862385321101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)