FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346
Sequences flagged as poor quality0
Sequence length43-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA381.1356843992827257RNA PCR Primer, Index 39 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC290.8667065152420802Illumina PCR Primer Index 11 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC230.6873879258816498RNA PCR Primer, Index 39 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT220.657501494321578RNA PCR Primer, Index 39 (95% over 23bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT150.4482964734010759Clontech SMART CDS Primer II A (96% over 26bp)
CCTCTCATCAGTGTTTTACTCTGCTGACTTGTGCCTCCTTCCTAAAACACT150.4482964734010759No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGC140.41841004184100417TruSeq Adapter, Index 11 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCTTGA120.35863717872086076RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC120.35863717872086076RNA PCR Primer, Index 39 (95% over 21bp)
CCCTGAAGTCAGCTGTAGCTGCAAAGGATTCCATTTCTGTAATAGAGAAA120.35863717872086076No Hit
CAGCATGTAGGTGGCAATTGAGGCCTTTGAAGGAGATGAGATCATCTCC110.328750747160789No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG90.26897788404064554Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG90.26897788404064554No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC80.23909145248057379Clontech SMART CDS Primer II A (96% over 26bp)
GAGGACTATGAGCCTACCAAAGCAGACAGCTATCGGAAGAAGGTAGTGCT80.23909145248057379No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC80.23909145248057379Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA80.23909145248057379Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCAAAGCAGACAGCTATCGGAAGAAGGTAGTGCTAGATGGGGAGGAAGTC60.17931858936043038No Hit
GGAAGTGAGGAGTGTGCACGGATGCTGAATGTTTGGGAATGAGAGGATG60.17931858936043038No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTC60.17931858936043038TruSeq Adapter, Index 11 (95% over 22bp)
CTGATAGGTCGACCATATTGTTCTCCTGTGACAGAGGTTACACCTGTCACT60.17931858936043038No Hit
CCAAAGTAGAGCCGGTACATATTGAATTTTGATTCATCTGGAAGGATCTCG60.17931858936043038No Hit
CTCATCAGTGTTTTACTCTGCTGACTTGTGCCTCCTTCCTAAAACACTCTT60.17931858936043038No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA60.17931858936043038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGACTTGTGCCTCCTTCCTAAAACACTCTTACCTTGACTTCCCCATAGC60.17931858936043038No Hit
AATGGTGAGTTGGCTGTATGAGTAGACAGTGCAACAGAGTATGGAGCTGG60.17931858936043038No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT60.17931858936043038Illumina PCR Primer Index 11 (96% over 27bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC60.17931858936043038Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCTTTGCCTTGAGCAGCAGGAGTAGCCCTTGGTAGAATCTAGTTCCCC50.14943215780035865No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCTT50.14943215780035865TruSeq Adapter, Index 11 (96% over 27bp)
CCCATGAGTGAGTTTTTAGGAGTGGGTCTGAAATTAGGATAGACATTTATT50.14943215780035865No Hit
CCGATGTTATGGTTTTCTACGAAGTCAGTGCTTACTTAGCTCACTAACAGC50.14943215780035865No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG50.14943215780035865Illumina PCR Primer Index 11 (96% over 29bp)
GAGATTAAGTGGCTCAGTCGCGGGCTCGTGCTAAAGAGATTTTTCGAATCC50.14943215780035865No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC50.14943215780035865Clontech SMART CDS Primer II A (96% over 26bp)
GCCTCAACTGAGCTCCATAGATTGGATCCGAGACCTGGCCTTCTTGGTTG50.14943215780035865No Hit
GTTTTTAGGAGTGGGTCTGAAATTAGGATAGACATTTATTGTTCTTGCAT50.14943215780035865No Hit
CTTCCTTCTCTATTTTTCCATTTGCACCATCACACCAGGGGAAATTATGG50.14943215780035865No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT50.14943215780035865RNA PCR Primer, Index 11 (95% over 21bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT50.14943215780035865RNA PCR Primer, Index 39 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTC50.14943215780035865No Hit
GCCATTGTCCCCATCCTGGACTGACCCTCAGTATTTTTCCTTTGAGTGGTG40.11954572624028689No Hit
GAGGGAGAGGAGGCTGCAACACCAAGCAGAGGAGGCAGGAATCGGAGTGCG40.11954572624028689No Hit
ACCTCAGCCTCAGCCTGTTCCTGGGAACACCTTTCTCCCTCAACTTCTCAC40.11954572624028689No Hit
CATGCTGGGGGAGTTGGTGAGGGAACAGTAGGGCCATTATGTTGCAGGGGC40.11954572624028689No Hit
CCCAGCATGGGGATCAGAAATATGGCAGTACTCCTCAGTGAAGTATATGCT40.11954572624028689No Hit
ACAGGGCACAGGAATGACATCTCCCATTCCAGTGCCCCTTCTTCTTCTTTC40.11954572624028689No Hit
GCTTTTGCAGGGCAGTGGCTGGGCGCTCCTGAGCACAGTCCAGCTTCTCAT40.11954572624028689No Hit
GGTCCAGTACGGCCCCTTCGTGATGAACACCCAGGCCGAGATCAGCCAGAC40.11954572624028689No Hit
CCTAATCCACTTTGTTTACTTTGTTCTGTATCCAGGGAGAGATGCCTAAA40.11954572624028689No Hit
GCCTCTGAAGTTCTTTAACCCAATTTTTTGCTCTTGCAAAGGACTCCTCAT40.11954572624028689No Hit
CCCACAATTAAGGTACAATCTGGTCACATAACTTTTTCCCTAGGAAGAAAT40.11954572624028689No Hit
CGGGCGGCCGACCTGTCGAGCTTTGCCAGGGCCGGCCGGCTCCTCGGCATC40.11954572624028689No Hit
GCTATCTGTGTGGGACAAGTTTAAATAGGACTATTCAGATAAACCAAAGCT40.11954572624028689No Hit
GCCAGCGCAGCCATTTTGGCTTCCTGACCTTGGGCTACGGCTGACCGTTTT40.11954572624028689No Hit
CACAGGGCACAGGAATGACATCTCCCATTCCAGTGCCCCTTCTTCTTCTTT40.11954572624028689No Hit
GCCTCAGGGTCTACAGGATTATAGCTCAGATACATTATAAAAAGATC40.11954572624028689No Hit
TAAATCTTCATGGGCGTCCTAACTGTAACAAAAACCCCACAATTTGAAC40.11954572624028689No Hit
GCCTCTCCCTGGCTCGAATGCCCCCTCCACTCCCGACCCGGGTGGATTTCT40.11954572624028689No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG40.11954572624028689RNA PCR Primer, Index 39 (96% over 26bp)
GACGCTGGAAGGGAAGTTTGCGAATCAGAAGTTCAGTGGACTTCTGATAAC40.11954572624028689No Hit
GATGATACGGTTCTCTAACTTGTAACAAATGCACAGAACCCTGTGACCATG40.11954572624028689No Hit
CCGAGACCTGGCCTTCTTGGTTGACATGACGATGCATCTGAACGCTTTGA40.11954572624028689No Hit
ATTTAGATGGTATTCTGGATGATGCAAAAAACAATGGGGTGGACAACAGC40.11954572624028689No Hit
GAAAAAGGAAGAGAAAAAGTTTGATATGCCAAGGACAGATTTTGAAAC40.11954572624028689No Hit
GAGTAGAGCTCGCTGCAGATCCGGGCTCTGACCATGATTTGGCGCCGCGCG40.11954572624028689No Hit
TCAGTATCCTCTTCTCCCCGACCCCAGCATGAGCTTTAATTGGATGTATTT40.11954572624028689No Hit
GCGAGCTGATCGTCTCCAGTGAGAAGTTGAGGGAGAAAGGTGTTCCCAGG40.11954572624028689No Hit
GCAGCAGTGCCGCGCAGAGACTCCAGGTCGCAGGTCAAGGACTGCAACTGG40.11954572624028689No Hit
GTCCGCTCCTGGCTCCTCCTCAAATGAGGCACTGCAGTCATGAAAACACTC40.11954572624028689No Hit
CCTTCCTAAAACACTCTTACCTTGACTTCCCCATAGCACATTTATAGGATG40.11954572624028689No Hit
GCTTCCATAAAGGGTTGAGAAATCCTGGGCAGGATTATCTTTAAGAAGGTT40.11954572624028689No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC40.11954572624028689No Hit
CCTAGCTTTGGTTTATCTGAATAGTCCTATTTAAACTTGTCCCACAC40.11954572624028689No Hit
CCTTCATGGGCATGCTGGTGAAAGAATAAATACATCCAATTAAAGCTCATG40.11954572624028689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)