FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346
Sequences flagged as poor quality0
Sequence length45-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2005.977286312014345No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG180.5379557680812911Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT140.41841004184100417Clontech SMART CDS Primer II A (96% over 26bp)
CCTCTCATCAGTGTTTTACTCTGCTGACTTGTGCCTCCTTCCTAAAACACT120.35863717872086076No Hit
CCCTGAAGTCAGCTGTAGCTGCAAAGGATTCCATTTCTGTAATAGAGAAA110.328750747160789No Hit
AATGGTGAGTTGGCTGTATGAGTAGACAGTGCAACAGAGTATGGAGCTGG110.328750747160789No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA100.2988643156007173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTATCTGTGTGGGACAAGTTTAAATAGGACTATTCAGATAAACCAAAGCT90.26897788404064554No Hit
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC90.26897788404064554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAATTTGAACAGAAGAACAGAAGTATCTGGTTACAGAAGTGCATTCATAC80.23909145248057379No Hit
ACAGAGATTTCTGGAAAATGAGCCATCAAGACATTTGAATGAAGACTAC70.20920502092050208No Hit
GAGGGAGAGGAGGCTGCAACACCAAGCAGAGGAGGCAGGAATCGGAGTGCG70.20920502092050208No Hit
GATGATACGGTTCTCTAACTTGTAACAAATGCACAGAACCCTGTGACCATG70.20920502092050208No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC70.20920502092050208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCAATAGCCAGGGTTGGCTCATTATCTTGTCAAGACCTAGAAGACTCCCT70.20920502092050208No Hit
AGCAGAGGAGGCAGGAATCGGAGTGCGAGCAGTAGCTGAGTGGGCACCATG60.17931858936043038No Hit
CCCAGAGGCACAGTTTTGCTAGGAACGCCCGGATCAGGTCATACATCTGCG60.17931858936043038No Hit
CAGCATGTAGGTGGCAATTGAGGCCTTTGAAGGAGATGAGATCATCTCC60.17931858936043038No Hit
GGTTTGGGAGTGGAGATCTGTGTGTCTGCAAGGCCACTGAGCTGAGC60.17931858936043038No Hit
GAGAAAGGAGCAGGACTTGGACGCCAACAGGAATGGCCCGAAGAGGAAGA60.17931858936043038No Hit
GCTCTTTGCCTTGAGCAGCAGGAGTAGCCCTTGGTAGAATCTAGTTCCCC50.14943215780035865No Hit
GAGTCATGGAGCTTGGTGCAGAGAGACATTGTGAGCTCAAATCAGAAGAGG50.14943215780035865No Hit
GCGTCCAGCCTGGGTGTCGTTACTTGCGGCTCCGTGGTGAAGCTACTCAAT50.14943215780035865No Hit
CCATGATAGTGGGTTTTTCTTTTGAGAATTTCCTTACTTTTTAGCACTAC50.14943215780035865No Hit
ACAAAAGAACTGAGGAAACTGAAGAACCAAGCTTGATCTGTTACCATTGGG50.14943215780035865No Hit
CCGATGTTATGGTTTTCTACGAAGTCAGTGCTTACTTAGCTCACTAACAGC50.14943215780035865No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC50.14943215780035865No Hit
GGCAGCATGGGGGAGAAGTCAGAGAACTGTGGGGTTCCAGAGGATCTGTT50.14943215780035865No Hit
CCTGCAAAGCACCGATAGCTGCGCTCTGGAAGCGCAGATCTGTTTTAAAGT50.14943215780035865No Hit
GGTGAGAGGGGATAAAAGAGCAAACACTAAGGCTGTCTCCAGAGGTGCTTG50.14943215780035865No Hit
CACAGATAGCAGCCTCTTGCTTGCCCTTCTGTTTGTACACTTGTCTATTTT50.14943215780035865No Hit
AATTGTGGGTGGGATATTCAGAAGCTGCTAAGTATGAATGTGCTCAAGA50.14943215780035865No Hit
CATACCTCTCTCACTCTCATCAGCAGTTTTTTCCGCTTCTTCCAGCTTTTG50.14943215780035865No Hit
GCAAAAAGCCATGATTGTGCCACTGCACTCCAGCCTGGGTGATAGCACA50.14943215780035865No Hit
GCTGTTCCGCAGGACCTCCACCTTCGCCCTCACCATCATCGTGGGCGTCAT40.11954572624028689No Hit
GATTCCAGAGGGTTAACCAGGAATGGAGACCATCCGTCGGCCCTGCTAAGG40.11954572624028689No Hit
GGAAGTGAGGAGTGTGCACGGATGCTGAATGTTTGGGAATGAGAGGATG40.11954572624028689No Hit
GGTGATGATGGGGTTGCAAACTTTCTCCAGCTCTTTCTGTTGATGTTCAA40.11954572624028689No Hit
GCTCACGCGCAGGCGGCCGCGCGGCGCGGCCACGGCGTCCTGCATCATGG40.11954572624028689No Hit
GCTGTTGGCGGCTGCGGCTGCTGCTGTGGCAGGATTTTCAATGTGGTGTGT40.11954572624028689No Hit
CAGCAGGGTGGGCTGTCTCCCTCTGCTGCCTCTCTCCCATGCCTTTCCCTG40.11954572624028689No Hit
GAGATTAAGTGGCTCAGTCGCGGGCTCGTGCTAAAGAGATTTTTCGAATCC40.11954572624028689No Hit
GATTAACGATGAGGACAAACAGAAGATTCTGGACAAGTGTAATGAAATTAT40.11954572624028689No Hit
CCTGGCTCCTCCTCAAATGAGGCACTGCAGTCATGAAAACACTCCTCTTCT40.11954572624028689No Hit
ATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGC40.11954572624028689No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG40.11954572624028689No Hit
CATTTAGATGGTATTCTGGATGATGCAAAAAACAATGGGGTGGACAACAGC40.11954572624028689No Hit
CAAGGCTGTGGGGCCACAGAGCTCCGGCAAGACCTGGTGGAGCAAGTGCTT40.11954572624028689No Hit
GGAAATAGATGGCTAGAAACCTTCAGCATGTAGGTGGCAATTGAGGCCTTT40.11954572624028689No Hit
GCAAAAGCTGGAAGAAGCGGAAAAAACTGCTGATGAGAGTGAGAGAGGTAT40.11954572624028689No Hit
CCAGCATGGGGATCAGAAATATGGCAGTACTCCTCAGTGAAGTATATGCTG40.11954572624028689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)