FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059
Sequences flagged as poor quality0
Sequence length45-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1835.982347172278522No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT140.4576659038901602Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC120.3922850604772802Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG90.2942137953579601Clontech SMART CDS Primer II A (96% over 26bp)
CAGCATGTAGGTGGCAATTGAGGCCTTTGAAGGAGATGAGATCATCTCC90.2942137953579601No Hit
CCCTGAAGTCAGCTGTAGCTGCAAAGGATTCCATTTCTGTAATAGAGAAA90.2942137953579601No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA80.2615233736515201Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATGGTGAGTTGGCTGTATGAGTAGACAGTGCAACAGAGTATGGAGCTGG70.2288329519450801No Hit
GCAGAGGGACAAGGTGTCATCCAAGAATTCACTTGAGTCCTATGCCTTCA70.2288329519450801No Hit
CCAGGAAGCTGAGAAGTACAAAGCTGAAGATGAGAAGCAGAGGGACAAGGT60.1961425302386401No Hit
CCGATGTTATGGTTTTCTACGAAGTCAGTGCTTACTTAGCTCACTAACAGC60.1961425302386401No Hit
GCTATCTGTGTGGGACAAGTTTAAATAGGACTATTCAGATAAACCAAAGCT60.1961425302386401No Hit
TGATTGTGCCACTGCACTCCAGCCTGGGTGATAGCACAAGAGCTTGTCTC60.1961425302386401No Hit
GTCAATAGCCAGGGTTGGCTCATTATCTTGTCAAGACCTAGAAGACTCCCT60.1961425302386401No Hit
GCTCTTTGCCTTGAGCAGCAGGAGTAGCCCTTGGTAGAATCTAGTTCCCC50.16345210853220007No Hit
ACAGAGATTTCTGGAAAATGAGCCATCAAGACATTTGAATGAAGACTAC50.16345210853220007No Hit
CTTGTGAAGCTGTCCTTAACACTGGAGGCTGTGAAAACAACTGGTCTTTCC50.16345210853220007No Hit
GCTATTGACTGGTTTGATGGTAAAGAATTCTCCGGAAATCCTATCAAGGTC50.16345210853220007No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG50.16345210853220007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGACCATGACCACAATAAAAATTTAGAACTTTCCATCACTCCAAAATATC50.16345210853220007No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA50.16345210853220007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGGATCCTACGGTTCAAGGAGGCCACCTCAGCCTCAGCCTGTTCCTGGG50.16345210853220007No Hit
GCAGCAGTGCCGCGCAGAGACTCCAGGTCGCAGGTCAAGGACTGCAACTGG50.16345210853220007No Hit
CCTCAAATGAGGCACTGCAGTCATGAAAACACTCCTCTTCTCCCTGGTCCT50.16345210853220007No Hit
GGTCATCCTGAGGGTGCGACTCCGCCGCGATGGTGACCCGGTTCCTGGGCC50.16345210853220007No Hit
GCAAAAAGCCATGATTGTGCCACTGCACTCCAGCCTGGGTGATAGCACA50.16345210853220007No Hit
ACCAAAGCAGACAGCTATCGGAAGAAGGTAGTGCTAGATGGGGAGGAAGTC40.13076168682576006No Hit
TGTCTGTGACTAATAACACTTCATTTTTAAGTTGTTTCCTGTCCTATACTT40.13076168682576006No Hit
CTTTGACAGACCTCTCTCCAGAACCTCACGCTCTTATATCCAGGAGGACG40.13076168682576006No Hit
AGCAGAGGAGGCAGGAATCGGAGTGCGAGCAGTAGCTGAGTGGGCACCATG40.13076168682576006No Hit
GCTAACTTCCCTCTTTTTTTTCATAGAAAATTCACAAATGTCATGGTTTG40.13076168682576006No Hit
AGTATTAAGAGGGGGGCAGCACATTGGAAGCCCTCATGAGTGCAGGGCCCG40.13076168682576006No Hit
TCAGCATGTAGGTGGCAATTGAGGCCTTTGAAGGAGATGAGATCATCTCC40.13076168682576006No Hit
CCTAATCCACTTTGTTTACTTTGTTCTGTATCCAGGGAGAGATGCCTAAA40.13076168682576006No Hit
GCCAGGGCAACCCCATCTCTACAAAAAATACAAAAATTAGCTGGGGGTGGT40.13076168682576006No Hit
GTATGAGTAGACAGTGCAACAGAGTATGGAGCTGGAAATAGATGGCTAGA40.13076168682576006No Hit
GCTTATTGTCAGGCCTCTGAGCCCAAGCTAAACCATCATAATCCCCTGTG40.13076168682576006No Hit
CCAAAGTAGAGCCGGTACATATTGAATTTTGATTCATCTGGAAGGATCTCG40.13076168682576006No Hit
TCAGGCCTTCCAGGGACTCCAGCTTCGTGCCGTTCTTGCGCGCCAGGAAGG40.13076168682576006No Hit
CCATCGAGGCGGTTAAGGGCAATATCCAGGTTCTGCAGCAGCAGGCAGATG40.13076168682576006No Hit
CCTCTTGCTGCCTCTTGCGGTCAACCACCCACTACCTGACACATCCCTGTC40.13076168682576006No Hit
GAGATTAAGTGGCTCAGTCGCGGGCTCGTGCTAAAGAGATTTTTCGAATCC40.13076168682576006No Hit
CGGTTCAAGGAGGCCACCTCAGCCTCAGCCTGTTCCTGGGAACACCTTTCT40.13076168682576006No Hit
AAATCTTCATGGGCGTCCTAACTGTAACAAAAACCCCACAATTTGAAC40.13076168682576006No Hit
CCCCATAGCACATTTATAGGATGCTTCTTTTTCTTCTCCTTTGAAGATTCT40.13076168682576006No Hit
CTTCATATTTCCTATTTGCCTCTTCTGCAATGTGCTTAGCTTCTTTGAGTT40.13076168682576006No Hit
ATTTAGATGGTATTCTGGATGATGCAAAAAACAATGGGGTGGACAACAGC40.13076168682576006No Hit
GACAGGAACTGAGAGACAGCACAGTGAGGCAAGGGAGCAGCAGACTGC40.13076168682576006No Hit
GGAAGATTTGGGGCTCCGCGATACAGTTAGGATGGCTGTAGTACCTCTGCT40.13076168682576006No Hit
GCAAATCGACCGGTGGTAAAGCACCCAGGAAGCAACTGGCTACAAAAGCCG40.13076168682576006No Hit
GGTGAGAGGGGATAAAAGAGCAAACACTAAGGCTGTCTCCAGAGGTGCTTG40.13076168682576006No Hit
CCTCTCATCAGTGTTTTACTCTGCTGACTTGTGCCTCCTTCCTAAAACACT40.13076168682576006No Hit
CTTCCTTCTCTATTTTTCCATTTGCACCATCACACCAGGGGAAATTATGG40.13076168682576006No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC40.13076168682576006No Hit
GCTGCTGTGGCAGGATTTTCAATGTGGTGTGTTTTCAAGCCTCACTCACTC40.13076168682576006No Hit
CCTCTCACCTGGGAATACAATTGCCCCTGCAACATAATGGCCCTACTGTTC40.13076168682576006No Hit
TCAGATGCATCGTCATGTCAACCAAGAAGGCCAGGTCTCGGATCCAATCT40.13076168682576006No Hit
CACCTGCTGGAGGGAGGAGTGGGGAGAGGGGAAGAGGCAGGAAGACAGAC40.13076168682576006No Hit
GTCTCCAGAGGTGCTTGGCTAACAGTGAGAAGATAAAGCTGCTTTGATTTC40.13076168682576006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)