FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007546494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC510.749669263560194Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTAT350.5144789063648391Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG310.45568131706600035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA310.45568131706600035Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG270.3968837277671615Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG210.3086873438189034No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTA200.29398794649419374Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT200.29398794649419374No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT200.29398794649419374No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT190.27928854916948403Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTA190.27928854916948403Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT180.26458915184477433No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT160.235190357195355Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG160.235190357195355No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT150.2204909598706453No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATA150.2204909598706453Clontech Universal Primer Mix Long (96% over 26bp)
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA150.2204909598706453No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC140.2057915625459356No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC130.19109216522122593No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCCTGTCTCTTATA130.19109216522122593No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA130.19109216522122593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG130.19109216522122593No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCC120.17639276789651626No Hit
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA120.17639276789651626Clontech Universal Primer Mix Long (96% over 26bp)
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC120.17639276789651626No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC120.17639276789651626Clontech Universal Primer Mix Long (96% over 26bp)
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT120.17639276789651626No Hit
CATGTTCAGACAGGATTTCACGTGTCCCGCCCTACTCGAGTCCTGACGATC100.14699397324709687No Hit
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATG100.14699397324709687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCCTGTCTCTTAT90.13229457592238716No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC90.13229457592238716No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT90.13229457592238716No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG90.13229457592238716No Hit
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA90.13229457592238716No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT90.13229457592238716No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG80.1175951785976775No Hit
GTTCTTCCGAGGGAATTCCCACTTTGGATCCTCGATGGTCACGCTGGCTTT80.1175951785976775No Hit
AGTTCTTCCGAGGGAATTCCCACTTTGGATCCTCGATGGTCACGCTGGCTT80.1175951785976775No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT80.1175951785976775No Hit
CCTTATGGTAAGTGGGTTTCCCCATTCAGACATCTCCGGATCACAGGATAT70.1028957812729678No Hit
GAGTACATGGGGGTTAAACACCCAAGCAGCAATACGTAACTGAACGAAGTT70.1028957812729678No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT70.1028957812729678Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)