FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007546495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG500.7349698662354843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG460.6761722769366456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT340.49977950904012935Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC300.4409819197412906Clontech Universal Primer Mix Long (96% over 26bp)
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA240.3527855357930325No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA230.3380861384683228Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT220.3233867411436131No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT220.3233867411436131No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG210.3086873438189034No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTA190.27928854916948403Clontech Universal Primer Mix Long (96% over 26bp)
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT190.27928854916948403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTA180.26458915184477433Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT180.26458915184477433Clontech Universal Primer Mix Long (96% over 26bp)
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG160.235190357195355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTAT150.2204909598706453Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA140.2057915625459356No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATA140.2057915625459356Clontech Universal Primer Mix Long (96% over 26bp)
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC130.19109216522122593No Hit
GTTCTTCCGAGGGAATTCCCACTTTGGATCCTCGATGGTCACGCTGGCTTT130.19109216522122593No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTCTCTTATA120.17639276789651626Clontech Universal Primer Mix Long (96% over 26bp)
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG120.17639276789651626No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT120.17639276789651626No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC120.17639276789651626No Hit
GAGTACATGGGAGGAGGAGAACCGCGACCTGCGCAAAGCCAGCGTGACCAT110.16169337057180655No Hit
CCTTATGGTAAGTGGGTTTCCCCATTCAGACATCTCCGGATCACAGGATAT110.16169337057180655No Hit
ACAGGGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGG110.16169337057180655No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC100.14699397324709687No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG100.14699397324709687No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCCTGTCTCTTATA100.14699397324709687No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC100.14699397324709687No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA100.14699397324709687No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG100.14699397324709687No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT90.13229457592238716No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80.1175951785976775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCAAAGCCAGCGTGACCATCGAGGATCCAAAGTGGGAATTCCCTCGGAA80.1175951785976775No Hit
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT80.1175951785976775No Hit
GTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATG80.1175951785976775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGTTAAACACCCAAGCAGCAATACGTAAC70.1028957812729678No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT70.1028957812729678No Hit
CTGGAAAGGCCGGCGCGATGGGTGACAGCCCCGTACGGGACGGAAGATCGT70.1028957812729678No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATA70.1028957812729678Clontech Universal Primer Mix Long (96% over 27bp)
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT70.1028957812729678No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC70.1028957812729678Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTCTCTTAT70.1028957812729678Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)