FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007546951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50515094
Sequences flagged as poor quality0
Sequence length35-51
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19713783.9025523737518926No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN752230.1489119271954636Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN621680.12306816651672467Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN523080.10354924807226926Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN510270.10101337235955653Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)