FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007546959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36663105
Sequences flagged as poor quality0
Sequence length35-51
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13034563.555225341661597No Hit
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN523890.14289297101268428Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN442570.1207126346772866Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN432080.11785144766107507Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN428920.11698954575724015Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)