FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007546961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37194868
Sequences flagged as poor quality0
Sequence length35-51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12632023.3961728268534253No Hit
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN534580.14372412882336347Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN449090.1207397751754355Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN431660.11605364482003269Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN427580.11495671929794185Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)