FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007546965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007546965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56189341
Sequences flagged as poor quality0
Sequence length35-51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9644591.7164447612937834No Hit
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN732280.13032364981821018Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN615140.10947627949578552Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN602580.10724098009976661Illumina Multiplexing Index Sequencing Primer (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)