FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007547351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92604373
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT71598797.731685630007991TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT59264376.3997377316079875TruSeq Adapter, Index 4 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT58082276.2720871723843965TruSeq Adapter, Index 4 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT42484604.587753107512536TruSeq Adapter, Index 4 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT38596094.167847451437309TruSeq Adapter, Index 4 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT27458322.9651213123596225TruSeq Adapter, Index 4 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT23454322.5327443229921767TruSeq Adapter, Index 4 (97% over 37bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT12341081.3326670868988013TruSeq Adapter, Index 4 (97% over 37bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT12132741.3101692292652314TruSeq Adapter, Index 4 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT10798611.1661015187695294TruSeq Adapter, Index 4 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT10423121.125553757596307TruSeq Adapter, Index 4 (97% over 37bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT8413580.908551046504035TruSeq Adapter, Index 4 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6483150.7000911285258635TruSeq Adapter, Index 4 (97% over 37bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6348800.6855831743496607TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6159350.6651251771879066TruSeq Adapter, Index 4 (97% over 37bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT6056510.6540198700983592TruSeq Adapter, Index 4 (97% over 37bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT5284320.5706339591543912TruSeq Adapter, Index 4 (97% over 37bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT4682470.5056424279229232TruSeq Adapter, Index 4 (97% over 37bp)
TGTTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3868060.41769733703612466TruSeq Adapter, Index 4 (97% over 37bp)
ACAGACGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3677010.3970665618566415TruSeq Adapter, Index 4 (97% over 37bp)
TCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3655040.39469410370069674TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGCAGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3420300.36934540877459426TruSeq Adapter, Index 4 (97% over 37bp)
CTATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3396600.36678613438697977TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3215760.3472578989331314TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3116290.3365165055434261TruSeq Adapter, Index 4 (97% over 37bp)
CGGTAGCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2975170.3212774843797063TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2818990.30441219012411TruSeq Adapter, Index 4 (97% over 37bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTC2792570.3015591931063558TruSeq Adapter, Index 11 (97% over 35bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTC2064880.22297867078048247TruSeq Adapter, Index 11 (97% over 35bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGAGATCGGAAGAGCACAC1951970.21078594204185153Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GGTAACGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1927350.20812732029404268TruSeq Adapter, Index 4 (97% over 37bp)
TAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1764690.1905622750666429TruSeq Adapter, Index 4 (97% over 37bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAAGATCGGAAGAGCACAC1739690.18786261853962338Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTC1707330.18436818313104933TruSeq Adapter, Index 11 (97% over 35bp)
CAAGAGCTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1657910.17903150210843716TruSeq Adapter, Index 4 (97% over 37bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTC1644900.1776266008517762TruSeq Adapter, Index 11 (97% over 35bp)
CCTTCGCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1613910.17428010662088278TruSeq Adapter, Index 4 (97% over 37bp)
TTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1525810.16476651701966602TruSeq Adapter, Index 4 (97% over 37bp)
NGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1478730.15968252384798287TruSeq Adapter, Index 4 (97% over 37bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTC1462360.15791478875409048TruSeq Adapter, Index 11 (97% over 35bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATAAGATCGGAAGAGCAC1424920.15387178313922606Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TATCTCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCT1410930.1523610553467059TruSeq Adapter, Index 4 (97% over 37bp)
CTATTGGATCGGAAGAGCACACGTCTGAACCAATAGAGATCGGAAGAGCAC1409760.1522347114212414Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTAGATCGGAAGAGCACAC1381760.14921109611097955Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1381270.14915818284304996TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGTTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1361040.1469736207813858TruSeq Adapter, Index 4 (97% over 37bp)
NCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1349620.14574041767984328TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1324380.14301484445016435TruSeq Adapter, Index 4 (97% over 37bp)
NTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1279980.1382202544581777TruSeq Adapter, Index 4 (97% over 37bp)
CTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCT1210240.13068929261040405TruSeq Adapter, Index 4 (97% over 37bp)
CCATAGTTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1182760.1277218301559042TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCT1143730.12350712638592133TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCT1130070.12203203405955787TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACGCAGCAAGATCGGAAGAGCAC1116760.12059473692457266Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
ATAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1017830.10991165611585103TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCT977000.10550257707592275TruSeq Adapter, Index 4 (97% over 37bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGAGATCGGAAGAGCACAC976060.10540106999050682Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGGATGGATCGGAAGAGCACACGTCTGAACCATCCTAGATCGGAAGAGCAC969890.10473479475963841Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
NAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT966580.10437736023546101TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)