FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007547353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112366228
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC70456926.270293241488893TruSeq Adapter, Index 7 (97% over 36bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC60535775.38736336330521TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC60083225.347088806789883TruSeq Adapter, Index 7 (97% over 36bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC40982223.647200829772447TruSeq Adapter, Index 7 (97% over 36bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC27166462.417671259731171TruSeq Adapter, Index 7 (97% over 36bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC23104582.0561854225452865TruSeq Adapter, Index 7 (97% over 36bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC16965611.5098495608484785TruSeq Adapter, Index 7 (97% over 36bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC12867921.1451768230575472TruSeq Adapter, Index 7 (97% over 36bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC8882450.7904910717479988TruSeq Adapter, Index 7 (97% over 36bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC8200910.7298376163343313TruSeq Adapter, Index 7 (97% over 36bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC8175490.727575370777775TruSeq Adapter, Index 7 (97% over 36bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC7179420.6389304088769447TruSeq Adapter, Index 7 (97% over 36bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC5483730.4880229671854785TruSeq Adapter, Index 7 (97% over 36bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC4377220.38954942938905096TruSeq Adapter, Index 7 (97% over 36bp)
CGGTAGCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3867380.3441763658739172TruSeq Adapter, Index 7 (97% over 36bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3679450.32745158981397865TruSeq Adapter, Index 7 (97% over 36bp)
TCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3662570.3259493590903488TruSeq Adapter, Index 7 (97% over 36bp)
TGTTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3524920.31369923710529823TruSeq Adapter, Index 7 (97% over 36bp)
CTATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3471940.3089842973103983TruSeq Adapter, Index 7 (97% over 36bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3469780.30879206873438875TruSeq Adapter, Index 7 (97% over 36bp)
ACAGACGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3158960.2811307326254646TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGCAGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3080810.2741757959517872TruSeq Adapter, Index 7 (97% over 36bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC3031070.26974919902090155TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2944440.2620395871969646TruSeq Adapter, Index 7 (97% over 36bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTC2800800.2492563868923321TruSeq Adapter, Index 10 (97% over 36bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2747590.2445209783138756TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2682900.23876391045181297TruSeq Adapter, Index 7 (97% over 36bp)
GGTAACGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2108350.1876319991803943TruSeq Adapter, Index 7 (97% over 36bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAAGATCGGAAGAGCACAC1954300.17392236393304936Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGAGATCGGAAGAGCACAC1900480.16913266858081238Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1780010.15841147573272638TruSeq Adapter, Index 7 (97% over 36bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTC1704290.15167279620705965TruSeq Adapter, Index 10 (97% over 36bp)
TAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1659970.14772855061042006TruSeq Adapter, Index 7 (97% over 36bp)
NGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1627810.14486648070094513TruSeq Adapter, Index 7 (97% over 36bp)
CCTTCGCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1625640.14467336217782445TruSeq Adapter, Index 7 (97% over 36bp)
NCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1622940.1444330764578126TruSeq Adapter, Index 7 (97% over 36bp)
ATCAAGCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1588310.1413511896118823TruSeq Adapter, Index 7 (97% over 36bp)
CAAGAGCTTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1583530.14092579489275017TruSeq Adapter, Index 7 (97% over 36bp)
TATCTCGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCT1571880.13988900650825442TruSeq Adapter, Index 7 (97% over 36bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTC1549340.13788306571971073TruSeq Adapter, Index 10 (97% over 36bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATAAGATCGGAAGAGCAC1535420.1366442593409828Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
NTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1466590.13051875337490193TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCT1449640.12901029302149397TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGTTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1381870.12297912144919557TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1339310.11919150654411928TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCT1275520.11351453392206064TruSeq Adapter, Index 7 (97% over 36bp)
CCATAGTTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1262100.11232022489889043TruSeq Adapter, Index 7 (97% over 36bp)
GTATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1260300.11216003441888252TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACGCAGCAAGATCGGAAGAGCAC1255320.11171684075752726Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
GCATTCGAATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1245500.1108429126943729TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGGATCGGAAGAGCACACGTCTGAACCTCAACAGATCGGAAGAGCAC1200720.10685772953062019Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGATGTTCGGAAGAGCACACGTCTGAACATCCTAGATCGGAAGAGCACAC1198220.1066352427528314Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTC1157750.10303362679398653TruSeq Adapter, Index 10 (97% over 36bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGAGATCGGAAGAGCACAC1146820.10206091460149397Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTC1129730.10053999498852983TruSeq Adapter, Index 10 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)