FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810046
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT13660.1686323986539036Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.12641257410073006No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT9970.12307943005705849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)