FastQCFastQC Report
Wed 8 May 2024
EGAF00007547402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734854
Total Bases37.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22460.30563894324586927Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.22956941106668807No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13700.1864315904928054Clontech Universal Primer Mix Long (96% over 26bp)
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTC10870.1479205393180142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.12519493668129994No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA8760.11920735275306388No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG7980.10859299942573626No Hit
GGTATCAACGCAGAGTGAATGGGTCGTCGGCAGCGTCCAAAAATATCCCC7960.10832083651990734No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAG7560.1028775784033291No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7530.10246933404458572Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)