FastQCFastQC Report
Wed 8 May 2024
EGAF00007547407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943181
Total Bases48.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG19220.20377849002471424Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.14292060590703162No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTC12900.13677120298224837No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12210.12945553398552345Clontech Universal Primer Mix Long (96% over 26bp)
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAG11790.1250025180744735No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA10960.11620251044073195No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT10120.10729647861863205No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG9710.10294948689594044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)