FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461797
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.24686171629525527No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC6490.1405379419961585No Hit
CATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCC6200.13425812640619147No Hit
GGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTG6000.12992721910276595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.12408049424314146No Hit
GCCGTTTACTGGGGCTTCGATCAAGAGCTTCGCTTACGCTAACCCCATCAA5680.12299776741728509No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT5310.11498558890594786Clontech Universal Primer Mix Long (96% over 26bp)
CCTGTCATAAGGCAGGTATCGTATGATGAATACATAGTCATACGAGGCGAA5260.11390286208009147No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG4880.10567413820358296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)