FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248941
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.2695417789757413No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT4570.18357763486127235Clontech Universal Primer Mix Long (96% over 26bp)
CATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCC4310.17313339305297237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.13336493385983025No Hit
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA3090.1241257968755649No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC3010.12091218401147259No Hit
CCCTTTAACCTTCCGGCACCGGGCAGGCGTCAGACTGTATACGTCGCCTTG2740.1100662405951611No Hit
GGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTG2700.10845943416311495No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC2690.10805773255510341No Hit
GCATCTTCACGGGCAATTCAATTTCGCTGAGTCCACGTTTGAGACAGCGGG2670.10725432933908034No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT2650.10645092612305727No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT2520.1012288052189073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)