FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27364
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA1048838.32772986405496No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA339412.403157433123813No Hit
CACCAAGTGCCATCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA8633.153778687326414No Hit
CACCAAGTGCCAACTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA8283.025873410320129No Hit
CACCAAGTGCCATCTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG5001.8272182429469375No Hit
CACCAAGTGCCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG3781.3813769916678846No Hit
AACTGGTCGGCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA3541.2936705160064317No Hit
GCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA3421.2498172781757053No Hit
TCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG3421.2498172781757053No Hit
CACCAAGTGCCATCTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA3271.1950007308872972No Hit
GTGCTGCTGAAAACCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCAC2410.880719193100424No Hit
AACTGGTCGGCTTCAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2380.8697558836427424No Hit
NACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA1950.7126151147493056No Hit
GTGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC1720.6285630755737465No Hit
AGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1530.5591287823417629No Hit
GCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA1450.5298932904546119No Hit
CACCAAGTGCCATCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1300.4750767431662038No Hit
TCGCAGTAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC1160.42391463236368954No Hit
CACCAAGTGCCAACTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG1150.4202601958777957No Hit
CACCAAGTGCCATCTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA950.3471714661599182No Hit
ACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG910.33255372021634266No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA900.32889928373044874RNA PCR Primer, Index 33 (96% over 29bp)
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCC720.26311942698435903No Hit
CACCAAGTGCCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG670.24484724455488965No Hit
CCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACG660.24119280806899576No Hit
CACCAAGTGCCAACTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA660.24119280806899576No Hit
NACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA650.2375383715831019No Hit
GTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC600.2192661891536325No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTGTCTCTTATACACATCTCCGAG500.18272182429469375No Hit
CACCAAGTGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG480.17541295132290602No Hit
AGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA450.16444964186522437No Hit
CACCAAGTGCCAACTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA440.1607952053793305No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCATCTGGTAGCTGCTGT420.15348633240754275No Hit
CCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAA380.13886858646396724RNA PCR Primer, Index 33 (96% over 29bp)
GCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC330.12059640403449788No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTGTCTCTTATACACATCTCCGAG310.11328753106271013No Hit
TCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG290.10597865809092238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)