FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230090
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA7360031.987483158763965No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA3008313.07444912860185No Hit
CACCAAGTGCCAACTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA60222.6172367334521276No Hit
CACCAAGTGCCATCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA52462.2799774001477684No Hit
AACTGGTCGGCTTCAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA32871.4285714285714286No Hit
AACTGGTCGGCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA27651.2017036811682384No Hit
GTGCTGCTGAAAACCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCAC21220.9222478160719718No Hit
CACCAAGTGCCATCTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG13840.6015037593984963No Hit
GCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGC12060.5241427267590942No Hit
NACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA12050.5237081142161762No Hit
GTGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATC11200.4867660480681472No Hit
TCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACG9890.42983180494589074No Hit
CACCAAGTGCCATCTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA9170.39853970185579557No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCC7600.3303055326176713No Hit
TCGCAGTAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGC7320.3181363814159677No Hit
CACCAAGTGCCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT7050.30640184275718196No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCATCTGGTAGCTGCTGT5780.25120604980659744No Hit
NACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA5360.2329523230040419No Hit
CACCAAGTGCCATCTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA4720.2051371202572906No Hit
GCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGC4670.2029640575427007No Hit
CACCAAGTGCCAACTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG4530.19687948194184887No Hit
CACCAAGTGCCAACTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA4260.18514494328306316No Hit
CACCAAGTGCCATCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4160.18079881785388324No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCC3800.16515276630883566No Hit
AGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA3620.15732974053631188No Hit
CACCAAGTGCCAACCCCATAAATATCGCTATACCTACAGTCACTGCCCAGT3520.152983615107132No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT3230.14037985136251033No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCAACTGGTAGCTGCTGT2970.12907992524664264No Hit
ACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACG2660.11560693641618498No Hit
CCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACG2340.10169933504280933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)