FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375908
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA4975113.234887259648637No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA205075.455324175064112No Hit
CACCAAGTGCCAACTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA46071.2255658299371122No Hit
CACCAAGTGCCATCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA42531.131393851687115No Hit
CACCAAGTGCCATCTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG33500.8911755003883928No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCATCTGGTAGCTGCTGT28630.7616225246602892No Hit
CACCAAGTGCCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT20170.5365674579950414No Hit
TCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT20140.5357693903827532No Hit
AACTGGTCGGCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA17880.47564829692371535No Hit
GCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG17800.4735201166242804No Hit
CACCAAGTGCCATCTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA17740.47192398139970415No Hit
AACTGGTCGGCTTCAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA17220.4580908094533769No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCAACTGGTAGCTGCTGT13550.36046053821679774No Hit
GTGCTGCTGAAAACCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCAC12640.336252487310725No Hit
CACCAAGTGCCAACTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG11970.3184289773029571No Hit
GTGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATC10630.2827819572874214No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT9970.2652244698170829No Hit
TCGCAGTAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA9370.24926311757132064No Hit
GCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG9090.24181448652329826No Hit
NACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA8470.22532108920267727No Hit
CACCAAGTGCCATCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8190.21787245815465486No Hit
AGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA8000.2128180299434968No Hit
CACCAAGTGCCATCTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA5830.15509113932132332No Hit
ACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT5780.15376102663417643No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCC5750.15296295902188833No Hit
CACCAAGTGCCAACTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA4790.12742479542866872No Hit
CCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACG4620.1229024122923694No Hit
CACCAAGTGCCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT4560.12130627706779318No Hit
GTTCTAAGTTGGTCGTTAAGCGCACGCCGGACGGCCGAAGCCTGCCAAGGG4170.11093139810804771No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA4060.10800515019632465RNA PCR Primer, Index 11 (96% over 28bp)
CACCAAGTGCCAACTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA4020.10694106004660714No Hit
CACCAAGTGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT3980.10587696989688966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)