FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413402
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA6434115.563785371139957No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA267766.476988500297532No Hit
CACCAAGTGCCAACTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA56181.358967784384207No Hit
CACCAAGTGCCATCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA49791.2043966889371605No Hit
CACCAAGTGCCATCTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG37710.9121871689058108No Hit
AACTGGTCGGCTTCAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA28130.6804514733842604No Hit
AACTGGTCGGCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA25380.6139302664234813No Hit
TCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT23790.5754689140352489No Hit
GCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT23390.5657931021136811No Hit
CACCAAGTGCCATCTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA23180.5607133008548579No Hit
CACCAAGTGCCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC22860.5529726513176036No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCATCTGGTAGCTGCTGT20220.48911229263525574No Hit
GTGCTGCTGAAAACCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCAC17280.4179950750117319No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT13420.32462348996860196No Hit
CACCAAGTGCCAACTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG12880.31156114387448536No Hit
GTGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATC11800.28543645168625215No Hit
NACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA10980.265601037247038No Hit
GCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT10880.263182084266646No Hit
CACCAAGTGCCATCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10180.24624941340390225No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCAACTGGTAGCTGCTGT9690.2343965437999816No Hit
AGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9340.22593020836860975No Hit
TCGCAGTAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA8990.21746387293723785No Hit
GTTCTAAGTTGGTCGTTAAGCGCACGCCGGACGGCCGAAGCCTGCCAAGGG6620.16013468730194821No Hit
ACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT6560.15868331551371306No Hit
CCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACG5840.14126685405489087No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA5820.14078306345881247RNA PCR Primer, Index 39 (96% over 30bp)
CACCAAGTGCCAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC5710.13812221518038134No Hit
CCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAA5410.13086535623920542RNA PCR Primer, Index 39 (96% over 30bp)
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT5200.1257855549803823No Hit
CACCAAGTGCCATCTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA5120.12385039259606873No Hit
CACCAAGTGCCAACTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA4750.11490026656861844No Hit
CACCAAGTGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT4700.11369079007842245No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCTG4690.11344889478038325No Hit
CACCAAGTGCCAACTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA4560.1103042559058737No Hit
NACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA4430.10715961703136415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)