FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007547641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007547641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648770
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA10769516.59987360697936No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA470687.254959384681782No Hit
CACCAAGTGCCAACTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA103111.5893151656210982No Hit
CACCAAGTGCCATCTGGTAGCTCCTGTCTCTTATACACATCTCCGAGCCCA95181.4670838663933288No Hit
CACCAAGTGCCATCTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG44710.6891502381429474No Hit
AACTGGTCGGCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCACGAGA41540.6402885460178491No Hit
AACTGGTCGGCTTCAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA40400.6227168333924195No Hit
TCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA29140.4491576367587897No Hit
GCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC27650.4261911000816931No Hit
GTGCTGCTGAAAACCTTCAACGCTGTCTCTTATACACATCTCCGAGCCCAC27450.42310834348074045No Hit
CACCAAGTGCCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC26950.41540145197835904No Hit
CACCAAGTGCCATCTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA24970.3848821616289286No Hit
GTTCTAAGTTGGTCGTTAAGCGCACGCCGGACGGCCGAAGCCTGCCAAGGG24480.3773294079565948No Hit
GTGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC23280.35883286835087935No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCATCTGGTAGCTGCTGT23220.3579080413705936No Hit
TCGCAGTAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG22670.349430460717974No Hit
NACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA18820.29008739614963697No Hit
CACCAAGTGCCAACTGGTAGCCTGTCTCTTATACACATCTCCGAGCCCACG16190.2495491468471107No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT15270.23536846648272886No Hit
GCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC13410.20669883009386994No Hit
CACCAAGTGCCATCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11860.18280746643648751No Hit
CACCAAGTGCCATCTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA11690.18018712332567782No Hit
GCGTCAGATGTGTATAAGAGACAGCACCAAGTGCCAACTGGTAGCTGCTGT11110.17124712918291538No Hit
AGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10470.16138230805986714No Hit
CACCAAGTGCCAACTGGTAGCTACTGTCTCTTATACACATCTCCGAGCCCA9000.13872404704286573No Hit
CTTTCTGGCTATCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT8540.13163370686067483No Hit
ACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA8360.1288592259198175No Hit
NACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA8090.12469750450853152No Hit
CTGGAGCAGGTGGGCACTAGCCGGGCAACCGGCCGGCGCCCGCCAGCATCG7910.12192302356767422No Hit
CCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGGGCACGTTGGGCCTT7380.11375371857514989No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCT6680.10296407047181588No Hit
CACCAAGTGCCAACTGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA6640.10234751915162539No Hit
CCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCACG6550.10096027868119672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)