FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18638174
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1260770.6764450208480723No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1019460.5469741832005646No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG640860.3438426961782844No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG621960.3337022178245573No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC540580.2900391422464454No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA406320.21800418860774667No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG325840.17482399295124082No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG325730.17476497429415563No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC324860.17429819036993643No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA316930.17004348172734088No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT285490.15317487646590272No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC283070.15187646601002866No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG280020.15024003960903037No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA256480.13761004699280088No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG253790.13616677256044502No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC248640.13340362634236594No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT232010.12448107845757853No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC228370.12252809744130513No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC216220.1160092184996234No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT208820.11203887247752918No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT205790.11041317674145545No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG203210.10902892096618477No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA194120.10415183375796364No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC194040.10410891109826532No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT193650.10389966313223602No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT192170.10310559392781718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)