FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007661168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25193222
Sequences flagged as poor quality0
Sequence length88-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA952110.3779230778818208No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC635100.25209161416511155No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT599790.23807593963169937No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA589010.23379701095794736No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT464740.18447025156210667No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA462790.18369623385210515No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG364310.14460635483623333No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA349280.13864046448683698No Hit
CGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGG258000.10240849701558619No Hit
GCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGAGAGTGG257060.10203538078614954No Hit
GGCCCATACCCGGCCGTCGCCGGCAGTCGAGAGTGGACGGGAGCGGCGGG256330.10174562031009769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)