FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007661199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25941180
Sequences flagged as poor quality0
Sequence length87-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1042310.4017974510026143No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG709380.27345710565209447No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG638240.24603352661675373No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC604020.2328421451915449No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA574240.22136232815932042No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG483990.18657208345958048No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA482280.18591289987579593No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA446950.17229362735234094No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC437270.16856210858565418No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT432320.16665394557996205No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC412610.1590559874300244No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA392270.15121517217027136No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT376690.14520927729578995No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG355530.13705236230580103No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG351500.13549884777793456No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA350900.1352675552923961No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT301330.1161589411121622No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG259900.1001881949857331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)