FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15240623
Sequences flagged as poor quality0
Sequence length87-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA994780.6527160995977658No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA796890.5228723261509717No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG498910.3273553843566631No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG488650.32062337609164665No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC394830.25906421279497566No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA369950.24273942082288893No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG327060.21459752662341952No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA307540.20178965124982096No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG298100.19559567873308065No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG260780.1711084907749506No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT257020.16864140002675743No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC251480.16500637802011112No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA250290.16422557004395424No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT239800.15734264931295788No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA206400.1354275346880505No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC202960.13317040910991632No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC201790.13240272395688812No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG199870.13114293293653417No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC198620.1303227564909912No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT188070.1234004672906088No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG175600.1152183870698724No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT175150.11492312354947694No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT171980.11284315608358003No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC167920.11017922298845657No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC165500.10859136138988545No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT161690.10609146358387055No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGT159240.10448391775060639No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT153380.10063893057390108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)