FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015088
Sequences flagged as poor quality0
Sequence length88-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA348900.8689722367230805No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG197860.4927911916251898No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG178250.44395041901945864No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA154030.3836279553524107No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG152080.3787712747516368No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA133610.33276979234328113No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC133190.3317237380600375No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA129540.32263302821756334No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT123210.30686749580582046No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA122190.30432707826080024No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC121580.30280780894466075No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG103500.25777766265645985No Hit
GGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA86810.21620945792470797No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA79980.19919862279481793No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT75570.1882150528207601No Hit
CAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGT71080.1770322344117987No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA67820.1689128606894793No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC65860.16403127403434248No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG59670.14861442638368078No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT59060.14709515706754123No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT56410.14049505266136134No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA55300.1377304806270747No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG52350.1303831945900065No Hit
TGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAA52060.12966091901348115No Hit
GCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG51860.1291627979262223No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGC51080.12722012568591273No Hit
CTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTC50290.12525254739124023No Hit
AAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGA50240.1251280171194255No Hit
GTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTT48300.12029624257301459No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCT48090.1197732154313928No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG47480.11825394611525326No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAA47000.117058455505832No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGA46340.11541465591787776No Hit
CCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGC46130.11489162877625597No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT45630.1136463260581088No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA45330.11289914442722052No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC45030.11215196279633223No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG43940.10943720287077145No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT42810.10662281872775889No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG42200.10510354941161938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)