FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18236396
Sequences flagged as poor quality0
Sequence length87-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1079730.5920742234375695No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG777690.4264493927418554No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG756000.4145555953051249No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC653370.35827802818056814No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA648970.35586527074757535No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA505650.2772751809074556No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC441450.2420708565442426No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA384640.21091886796053344No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT383250.21015665595329253No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC369480.2026058218959492No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG367490.20151459751148199No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA346830.19018560465565673No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC339360.18608940055918943No Hit
GTTTAGGACCTGTAGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG326280.17891693073565632No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT309570.16975393602990416No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT296780.16274048885536374No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA282640.1549867638320642No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT282080.15467968561331966No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC281900.15458098190015176No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG273250.14983772012847277No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT268040.14698079598622446No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC257900.14142048681110017No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGT248290.1361508052358591No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC241940.13266875757688087No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA239630.13140205992455964No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG235930.12937315026499752No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT228020.12503567042523095No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC223080.1223268018527345No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC220040.12065980580812129No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT217470.11925053612566869No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG204310.11203419798517207No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA201550.11052074104993113No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG196220.1075980144322376No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT187180.1026408946153615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)