FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12344864
Sequences flagged as poor quality0
Sequence length86-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA234190.1897064236592643No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA207160.1678106781897314No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG162600.13171469527732343No Hit
GCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGA161210.1305887209450019No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT152070.12318483216988052No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG149320.12095718510953218No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG140640.11392592093359635No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGG139880.11331028029146373No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT137710.11155246424747976No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA125730.10184802359912593No Hit
GGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGG124210.10061674231486065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)