FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13159797
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA626150.47580521188890684No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG410970.3122920513135575No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG401300.30494391364851603No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC346530.26332473061704526No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA331800.25213154883772143No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA261030.19835412354765047No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC246650.18742690331773354No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG235750.17914410077906215No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG223670.16996462787381902No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA221850.16858162781690328No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT205380.15606623719195667No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT205330.15602824268489854No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC190080.14443991803217024No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC172300.13092907132230078No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC169820.12904454377221777No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT163440.12419644467160093No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA160050.12162041709306003No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT158790.12066295551519525No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG157950.12002464779661876No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC155600.11823890596488684No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA151490.11511575748470892No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC137500.10448489440984539No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG135890.10326147128257374No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT134080.1018860701270696No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT133560.10149092725366508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)