FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591709
Sequences flagged as poor quality0
Sequence length88-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1405880.7991719280940812No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAG967260.5498385631549499No Hit
GTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA934880.5314321650045485No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC900470.5118718141597272No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA720100.40934055923730883No Hit
GCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG618130.3513757532028298No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC584880.33247480389767703No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT546410.310606547664016No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG497020.28253082176382066No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG471650.2681092553315883No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC462140.26270329960551303No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA461260.26220306395473003No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT425540.24189804412976587No Hit
TTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA423230.24058492554646055No Hit
GCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA398170.22633957849120856No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC395090.22458875371346806No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT378900.2153855546382674No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC374830.21307196475339607No Hit
GTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT347640.19761582004340794No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG346060.1967176696704112No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC330580.18791806981345588No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA310050.17624779946052996No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA305450.1736329312859825No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT301130.17117722900032056No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG300770.17097258714318206No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC294870.16761873448452336No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT291950.16595886164328888No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC286870.16307113765922343No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT276050.1569205129530053No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC276010.1568977749688788No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA271300.1542203773379835No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA265160.1507300967745658No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG257600.14643261777465738No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGG247650.14077654422319058No Hit
CTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTC246720.14024788609224947No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA240600.13676897452089504No Hit
GTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATT237520.13501814974315457No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT224990.12789547621552858No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT217070.12339335535848167No Hit
GGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAA212060.12054542284663759No Hit
GCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACA211440.12019298409267684No Hit
CGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG206350.11729957561257977No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG205230.11666291205703778No Hit
CAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATT203030.11541232293008029No Hit
GACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC201010.11426405473169206No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT196030.11143317570794288No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT190760.10843744629927655No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA187860.10678894245010533No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCT187210.1064194502080497No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG182790.1039069029620715No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCACGAGGGTTCAGCTG182280.10361699366445863No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA177280.10077474564864619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)