FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591709
Sequences flagged as poor quality0
Sequence length88-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1467390.8341372631846059No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAG944780.5370598160758572No Hit
GTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA876660.49833702910842825No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC837880.476292553497787No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA693350.39413453235271234No Hit
GCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG588800.334703126342074No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC558790.31764395375116766No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG518240.29459332234292873No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG494060.2808482109384597No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT488700.27780132106550876No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA475630.27037168475217505No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC475070.270053352974404No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT427000.24272798055038314No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT406090.23084169934825546No Hit
TTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA404250.2297957520784365No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC385940.2193874398445313No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC374640.21296395932879517No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG373200.2121453919002412No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC367730.20903597257094236No Hit
GCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA364440.20716577337653777No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA353350.20086166727746577No Hit
GTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT331700.18855473336899786No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC329160.18711087137696514No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG322570.18336478849212434No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT309740.1760715800835496No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA302500.17195600495665317No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT299760.17039845304398793No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC299450.17022223366700756No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA294430.16736861665913186No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC289040.16430467329808604No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT260050.14782531930240547No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA250300.1422829356715712No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT248150.14106076902477185No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGG242390.13778649931055592No Hit
GTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATT242230.1376955473740499No Hit
CTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTC237190.13483056137411095No Hit
GACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC214350.12184717243787968No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA211690.12033509649346746No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT211310.12011908564426572No Hit
GGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAA205610.11687892290623952No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCACGAGGGTTCAGCTG200660.1140650973705852No Hit
CAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATT198520.11284861521981748No Hit
CGTCTTGCTGTGCCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGG195500.11113189741826675No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT190510.10829533389848593No Hit
GCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACA189900.1079485796405568No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA188500.10715275019612934No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG187140.10637965873582834No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGCCATGCCCGCCTCT184540.10490168976760586No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA178750.10161036656529505No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG176270.10020061154945208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)