FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007661450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19545894
Sequences flagged as poor quality0
Sequence length88-101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG550310.28154762325018234No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA497210.2543807922011651No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT469530.24021925014020848No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC405010.20720975975823874No Hit
CGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGTGG389220.19913133674008465No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA362870.18565024449636328No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA298400.15266633493459034No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT285700.1461688066045994No Hit
GCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGA261710.1338951290741677No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGGAC247180.12646134272497334No Hit
CTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCC241140.12337117964519811No Hit
GGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGG231110.11823966711371708No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG216670.11085192624087699No Hit
CCCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACA214330.10965474385566606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)