FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007661458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22467163
Sequences flagged as poor quality0
Sequence length88-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1038670.46230581048439445No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA865730.3853312498778773No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG514760.22911660007985876No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG497910.22161676576610942No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC394630.17564745491008366No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC385550.17160600116712554No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG359240.15989557738108723No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA342410.15240464494782896No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG317210.14118827552904656No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA308190.13717352742756173No Hit
GCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGA280760.12496459833402197No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT271600.12088753706909947No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG258470.11504345252669418No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA243750.10849166848524666No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC233470.10391610191282273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)