FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13068946
Sequences flagged as poor quality0
Sequence length87-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA768250.5878438858037978No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG538610.4121296392226274No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG536040.410163145520687No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA466710.35711372592709467No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC460560.35240791415007755No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA357520.27356452463725844No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC314000.24026421105420437No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG309290.23666024788839132No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT278800.21333013389144007No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG256960.19661876328817945No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA256310.19612140106784437No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCT249670.19104065469395926No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC240030.18366439038006585No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT218880.16748098890300717No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC217690.16657043345347053No Hit
GCCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC209340.16018124185378071No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA207890.15907174151611No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC206000.15762556521390478No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG188040.14388306447972163No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT182180.1393991527702387No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA181540.13890944227637025No Hit
CACCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTC167340.1280439906936642No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA163000.12472314140711882No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG162410.12427168954558386No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT161740.12375902387231534No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT157370.12041521940636987No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG156090.119435798418633No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC153450.11741574263142568No Hit
CCACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGA153240.1172550563756251No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT152220.11647458027602227No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG150920.115479855835352No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC150020.11479120045334949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)