FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36858
Sequences flagged as poor quality0
Sequence length94-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA6061.6441478105160345No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4921.3348526778447012No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.41781974062618693No Hit
CAGTAGACCTGGCGTCAGTAGACCTGTCTCTTATACACATCTCCGAGCCC1430.3879754734386022No Hit
CAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTCCGAGCC1300.35270497585327476No Hit
AAGGAGACACCGTAAGTCTTGAGAGAACGGGCTAGCTTCACGTAGCGGAC1280.347278745455532No Hit
GAGCTACAGGGGTGACTGGGAGGAGGATGTTGTGTTACACACGCATCAGA1270.3445656302566607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.331000054262304No Hit
CAACTCGGGGGCCTGGAGGCAGGGGTGGTCTCTCTTGAGCTCTCCTTGCG980.2658852894893917No Hit
GGTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCC920.24960659829616366No Hit
CCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTA850.23061479190406423No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTAT830.22518856150632155No Hit
GTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCCC800.21704921590970755No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA710.19263117911986544No Hit
GCTCCGGGGTGTGTGGACGCCGCTTTGTTGCCTGAGGTGGGTGGCGGTGG680.18449183352325138No Hit
GCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGACGACTTCCG670.18177871832438006No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTAT650.17635248792663738No Hit
GTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTCCGAGCCCA630.17092625752889468No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT590.1600737967334093No Hit
TGCGGGTGGAGCCCCCGACCAGGACCAGGTCGTGAATCTGGGCCTTGTCC550.14922133593792394No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA550.14922133593792394No Hit
GCCCAAGCCCATGAGGGCCGCGCGCCCGGCCGCCGGTGCTGACGAGACGG540.1465082207390526No Hit
ATCCTATGGGTTTTAAATGCTTTCCTGCAGCCACCTGGACGTCAAATGAT540.1465082207390526No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCTGTCTCTTA530.14379510554018124No Hit
GTTGGGCAGTGGGGCAGTGGGGTCCGGCCTTGATCACCTCCAGGCTGGTG530.14379510554018124No Hit
ATCCCCAGGCTCCTGGCTGGTGCCCCCCGGGAGCTCGCTGTGCCCGATGG510.13836887514243856No Hit
TCCTCATAGAGGACGCTCGGTGGGACACGAACACAGGGAGCCTCAGACTT510.13836887514243856No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT500.1356557599435672No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG500.1356557599435672No Hit
GCACATGATCATTCCACAAGTGAGTTCTGCGGCACTGAGGCTGTTCCCAT490.13294264474469586No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG490.13294264474469586No Hit
GCCTAACGGTATCGAGCGAGCCGGTTGTAGACGTGGTCCAGGTTTCTGCA480.1302295295458245No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA480.1302295295458245No Hit
GCCTGATGGGGTGGCTGGGGGTGCAGTGCCAGGCAGGACTTGCCGACGTG470.12751641434695316No Hit
GCTCTACAAAGCCCATTCACACACATCCTCTTAATCTTCACAGGAACCCT460.12480329914808183No Hit
GTGCTGTGGTCACCAGCCCCCAGGCTGGAAGCTGCCTCTGACCTGGCACC460.12480329914808183No Hit
CTGATACACCTCCCGGATGTCCAGCTCCAACAACTCAGCACTGACCGGCT450.12209018394921049No Hit
CATCTGTGTAACCTGTGACCAAGCAAGGGCTGGAAGAGTGGGCGATCGTC440.11937706875033915No Hit
CGTAGGAGGCGTAGAGCTCCAGGTTGATCTGGCGGTTGATGGCGGCCTCT440.11937706875033915No Hit
CGCCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTGTCTCTT440.11937706875033915No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT430.11666395355146779No Hit
CCCCTCCTGGGAGGGCAGGGACTGGCCCCGCACATTCCCAGGACTTGGAA430.11666395355146779No Hit
CCCTTTCTGCTCTGGCTCAGGTGCGGTTCCTGGAGCAACAGAATAAGGTG430.11666395355146779No Hit
TCCGGAGGCTGGGGAGATGACATCAGAAAACCTGGTCCAAACTGCTCCAA420.11395083835259645No Hit
GGGATGAAGGATGACCCCAGTGTCACCAGGGACCAGAAGGCAATGCCCCC420.11395083835259645No Hit
TTCTAATTCCGTGTTCATTTTTTTTTCTACAGACATTTGCCATGGCTGAG420.11395083835259645No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG420.11395083835259645No Hit
TCAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTCCGAGCCCA420.11395083835259645No Hit
ATTTATGCTTACTTTTCCATTTCCTCCTATGCTTTCTTCCTTATAACTGG410.11123772315372511No Hit
GAAAACATACTCACCGGTTCCTGAACAAAGGCTGAGCCAGCTTCGAGGTG400.10852460795485377No Hit
CTACTGACGCCAGGTCTACTGACTGTCTCTTATACACATCTCCGAGCCCA400.10852460795485377No Hit
AGGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTA390.10581149275598242No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAATTGTAAATCTCGTATGCC390.10581149275598242No Hit
GGCAAGATCACCTTATCTAGGATTGTGGAGTCGTTGGTCGCCGACAAGTT390.10581149275598242No Hit
AGCAAATGGCTTTCCAAAAGCAGAAACAATCTACCTGGACACCTGCTTCG390.10581149275598242No Hit
GGACGGTAAGGAGCCATCGGACAAACCTCAAAAGGCGGTGCAGGACCATA390.10581149275598242No Hit
CTCGTACGGCAGCTGTCAGCAATTCCCGCATGTGCACCAGTGCCTGCTTG380.10309837755711107No Hit
CAGTAGACCTGGCGTCAGTCTGTCTCTTATACACATCTCCGAGCCCACGA380.10309837755711107No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC380.10309837755711107No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA370.10038526235823973No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC370.10038526235823973No Hit
CCTCGGAGGTCTACCAGGGCTGCGGCACAGAGCTGCGAGTCATGGGTGTG370.10038526235823973No Hit
GGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAATTCGGCCAGGGTTC370.10038526235823973No Hit
CTCTCTGGGCAGGCTGGTCAAACTCAAAGGGCATAGCTTCTTCTGGTGGA370.10038526235823973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)