FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36858
Sequences flagged as poor quality0
Sequence length93-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA5181.4053936730153562No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4101.112377231537251No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.3852623582397309No Hit
GTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTGC1420.3852623582397309No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1380.37440989744424547No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1270.3445656302566607No Hit
GGTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTG1270.3445656302566607No Hit
GGAAGTCCTTCAGGTCAAGGAAGCCAGCCTTGTGCTTCTGCTCATTGTGG1230.3337131694611753No Hit
GATATCAGCTGCCTGCTGTTCCCCAGATGTGCCAAGTGCATTCTTGTGTG1210.32828693906343265No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.2984426718758479No Hit
GTCACCGCCTCTAGCATCACTTCTGTCCTGACTCAACAGGCCTACGTCCT970.26317217429052037No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG950.25774594389277766No Hit
CAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTGACGCTG880.2387541375006783No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCTGTCTCTTA830.22518856150632155No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTAT810.21976233110857887No Hit
CAGTAGACCTGGCGTCAGTAGACCTGTCTCTTATACACATCTGACGCTGC800.21704921590970755No Hit
CCGCCGCTCATGGCTGCTGCCGGCGCGACTCCTACCCTAAGGGCTAACTG680.18449183352325138No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG660.1790656031255087No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT660.1790656031255087No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTAT640.173639372727766No Hit
GGTCCGAGCTCCGGGAAGCGGGGCCCGGCGCCCAGGGTGAAGTCATCCGG620.16821314233002332No Hit
GGTCTACTGACGCCAGGTCTACCTGTCTCTTATACACATCTGACGCTGCC620.16821314233002332No Hit
GGAACAGCAGCCCGGGGCGTGAGGCGCAGAACCCGGCTGCGGAGCTCATG590.1600737967334093No Hit
GCCAAGAGGACCCTGTCGTCCAGCACCCAGGCCAGCCTGGAGATCGACTC560.15193445113679527No Hit
CCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTA530.14379510554018124No Hit
GTCGTTGGGAGGAAGAGTCAGGTAGATGTTTTTATCATCGTCAGTAGGTG530.14379510554018124No Hit
GTGAAATACTGTAGAAACGATGAAGAGGGCACAGAGTCCCATTCCATAAA520.1410819903413099No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA520.1410819903413099No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT500.1356557599435672No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG490.13294264474469586No Hit
GGGTCTGTGCCCATTGATGTCCCCCTTTTCTTTGATCTTTAGCACCCCCA470.12751641434695316No Hit
GTTCCCTTAGATACCGACTGCATTTTGAGAGAGACAGCAGGGAGGAGGTA460.12480329914808183No Hit
ATGAGAACAAGAGCACACTGCAGTTGGAGCAGCAAGTAAAGGAGCTACAG450.12209018394921049No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC450.12209018394921049No Hit
GCCTTGATGGGGCCATCCAGCTCCGCAGGGCCTGCGCGTCTCCGGCTTCC450.12209018394921049No Hit
CCATTGATGCGGGGCTTTGTACATTTCCACCACGCTGCTTACTCTTTTTT440.11937706875033915No Hit
CAGTAAACCTTCCCCATCGGGCTCCTTCTGGAGTGAATTGAGGCACTGGA440.11937706875033915No Hit
GTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCGCTGTCTCTT440.11937706875033915No Hit
CCTCATTTCACTCTCCTGAGAAGCCGGGAGCCTTCCTCGCTCAGAGCCCT440.11937706875033915No Hit
CCTCGGGAGTCCGCATCTCAGCCCCCAGGAAGAGGAAGACGATCGCCCAC440.11937706875033915No Hit
CCCCAGCCCCTTCCCCTCACTTTTCACTGTGATGTTCATCCGCTCACAGT440.11937706875033915No Hit
CTACTGACGCCAGGTCTACTGACTGTCTCTTATACACATCTGACGCTGCC430.11666395355146779No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA420.11395083835259645No Hit
TCACCGCACCCTCGGACTGCCCCAAGGCCCCCGCCGCCGCTCCAGCGCCG420.11395083835259645No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA420.11395083835259645No Hit
AGAGAAGCCTAGAAAGCTGAGTGCTCGGAGAACCGGCCTGGGAACAAACA420.11395083835259645No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT410.11123772315372511No Hit
CTCTGAAGTCTGTCCAGCGTGCTCCGCATGCTACCCAGGTAGGCCTCAAA410.11123772315372511No Hit
ATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAA400.10852460795485377No Hit
AGCTGGAGCGAAGCTTGCGGGACGTCGTAGAGAAAACCATCCAAAAGTAC400.10852460795485377No Hit
GTGAGATGGAGAATAAAGAAACTCTCAAAGGGTTGCACAAGATGGATGAT400.10852460795485377No Hit
TCAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTGACGCTGCC400.10852460795485377No Hit
GGTCTGTAGTCCCAGAGTACGGAAAAACAAAATAAGGTGAGTCATAAAGC390.10581149275598242No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC380.10309837755711107No Hit
GGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTT380.10309837755711107No Hit
ACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTGACGCTGCCGAC380.10309837755711107No Hit
GAGGTAGGGGTACCTGGGCTGAGCTGTGACTCTCTGCTTCCCCCTAGTGT380.10309837755711107No Hit
GGGCTGCACCGCCTTTTGAGGTTTGTCCGATGGCTCCTTATGGTCCTGCA380.10309837755711107No Hit
GCATGCGGGGTCTTGCGGCAGTTAGCAGGAGTCAGGTGTCCCTCCTTGTG380.10309837755711107No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTCTGTCTCTTA370.10038526235823973No Hit
TTTCTGTGCAGACAGGTCCAGCTGCAGAAATTAAGACTGGGGCTGAGTCG370.10038526235823973No Hit
GGCTAGGGTGGGGCGGACAGCTACAGGAGCGTCCCAGCCACCTGGCACAC370.10038526235823973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)