FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007661498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28627066
Sequences flagged as poor quality0
Sequence length87-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1087210.3797839429300928No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA988050.34514539492101637No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG762510.26635981486890764No Hit
CTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCC619140.2162778399993908No Hit
GGTCGGGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGAC594870.2077998492755073No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG579200.20232600854030938No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG574910.20082742674362788No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC480320.16778527006574825No Hit
GCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTT462940.1617140925304745No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT450180.15725677231470386No Hit
GGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGG446860.1560970306911648No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT429570.15005729193484238No Hit
CCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACAC424640.14833514548784008No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT410980.14356343748255584No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG409030.1428822639386097No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCC404590.14133128417700927No Hit
GCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGG394680.13786952529469837No Hit
CGCGTGTGGTGTGCGTCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGTGT383690.13403050106497116No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC380570.13294062339465734No Hit
GGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCG354120.1237011155806187No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA347720.12146546907741086No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT345100.12055025129016016No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG318220.11116053597668724No Hit
GTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCAC317530.11091950533806015No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC308130.10763589953647362No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA298370.10422653861908168No Hit
GAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGG293820.10263713368320736No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC292940.10232973228901628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)