FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007661505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21545666
Sequences flagged as poor quality0
Sequence length88-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA2534241.1762179920546434No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2469641.146235163953623No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG1474240.684239698136971No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1354420.6286275857056356No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1312920.6093661713682929No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT1031060.47854635823278796No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1028150.47719573857684416No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG923230.42849917008831384No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC889890.4130250603532052No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA800980.37175922062469546No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT771280.35797454578568144No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT680050.31563192337614443No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC659610.30614509665192063No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT658550.30565311835800296No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC657400.30511936832214887No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG644710.2992295527091156No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA626110.29059672604225834No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA622230.2887958998343333No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA619810.28767270410670986No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA615180.2855237800493148No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC605150.2808685514757353No Hit
GCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG583330.2707412247084866No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG555590.2578662455827543No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT555520.2578337564501371No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA533650.24768322315959043No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT528990.2455203751882165No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGG480460.2229961236751744No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC474450.2202066995747544No Hit
GCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG459990.2134953730369718No Hit
ACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC448940.208366731388113No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC436830.2027461114453366No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG433110.20101954611196518No Hit
TTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAA431340.20019803518721585No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC425910.19767780675705265No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA424260.19691199148821859No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT424090.19683308930900537No Hit
CGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCAC421480.19562170879284957No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTC419680.19478627395412143No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT416790.19344493690749684No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG409640.19012640407588235No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATC408120.18942092576762307No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAA405510.18820954525146727No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA395360.1834986210219726No Hit
GGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAAT363660.16878568525103843No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT358470.16637684813270567No Hit
GCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAG357170.1657734785269576No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCT344300.1598001194300515No Hit
CGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCC338000.15687609749450307No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT333770.15491282562349198No Hit
GGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGG330530.15340904291378138No Hit
GGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA326320.15145505365208947No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG320920.14894874913590508No Hit
CCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA318140.14765846644053612No Hit
GACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC315800.14657240115018957No Hit
GGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAAT311290.14447917274870967No Hit
CGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA307080.14252518348701776No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA296750.13773071577365026No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC292920.1359530960890232No Hit
AGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACG289350.13429615032554573No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT268990.12484645403859876No Hit
ATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGA268560.12464687793823594No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTAATTAAGGAACAAGT266920.12388570397406141No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG266670.12376967135757141No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG262590.12187601905645433No Hit
AGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCC262330.12175534513530471No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG261310.12128193206002544No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT252750.11730897527140725No Hit
TTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTT243370.11295543150070181No Hit
GGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCT237080.11003605086981297No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG225470.10464749616001659No Hit
CTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGT223220.10360320261160642No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG221500.10280489821015512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)