FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007661511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007661511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47182
Sequences flagged as poor quality0
Sequence length91-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCC3920.8308253147386715No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTAT3610.7651222923996439No Hit
CAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTCCGAGCC3590.7608833877326099No Hit
GTCTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCCC3280.6951803653935823No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTAT3140.665508032724344No Hit
CAGTAGACCTGGCGTCAGTAGACCTGTCTCTTATACACATCTCCGAGCCC2800.5934466533847654No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2360.5001907507100165No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCTGTCTCTTA2300.4874740367089144No Hit
CCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTA1850.39209868170064854No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTCTGTCTCTTA1610.3412318256962401No Hit
GTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTCCGAGCCCA1470.3115594930270018No Hit
CAGTAGACCTGGCGTCAGTCTGTCTCTTATACACATCTCCGAGCCCACGA1440.30520113602645077No Hit
TCAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTCCGAGCCCA1440.30520113602645077No Hit
ACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCCCACGA1360.2882455173583146No Hit
GTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCGCTGTCTCTT1350.2861260650247976No Hit
CGCCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTGTCTCTT1320.2797677080242465No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCACTCTATCTCGTATGCC1310.27764825569072954No Hit
AGGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGCTGTCTCTTA1290.27340935102369546No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA1270.26917044635666143No Hit
GGTCTACTGACGCCAGGTCTACCTGTCTCTTATACACATCTCCGAGCCCA1240.2628120893561104No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG1170.24797592302149124No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1120.23737866135390617No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG1110.23525920902038913No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG1070.22678139968632105No Hit
CTACTGACGCCAGGTCTACTGACTGTCTCTTATACACATCTCCGAGCCCA1000.2119452333517019No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC950.20134797168411683No Hit
CAGTAGACCTGGCGTCAGTAGCTGTCTCTTATACACATCTCCGAGCCCAC930.19710906701708278No Hit
CCTACTGAGCTAGAACCTACTCAGGATTCATTGGTGCAGTCTGAAAGTTA890.1886312576830147No Hit
CTGTCTAGACTTTCCTCTTTGCCTTTCGCCAAGTTTCTCTTAGTTTGTCC850.18015344834894664No Hit
GAGAAGGAAGGGGAGTTCACCCTGCACGGCCGGGCCATCCTGGAGATGGA800.16955618668136155No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCTACTGGCGCTGTCTCT790.1674367343478445No Hit
GGTTGAACCCGATCCATTTTCCAAATACTCCTGGCAGAAACGGAGTTGGT770.16319782968081048No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT760.16107837734729347No Hit
ATACACATCTCCGAGCCCACGAGACGCCGCACTCTATCTCGTATGCCGTC730.1547200203467424RNA PCR Primer, Index 32 (95% over 21bp)
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT690.14624221101267434No Hit
GGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCTTATACACATCTCC660.13988385401212325No Hit
GAGATATTCCGCCAAGCCAGATTCGGGCGCTCCCATCTTGCGCAAGTTGG660.13988385401212325No Hit
GAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGGGTTCA650.13776440167860624No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCCTGTCTCTTATACACATCTCC650.13776440167860624No Hit
GGACCAGGTCGTGAATCTGGGCCTTGTCCAGCTTGGCGTCGCGCAGAGCC640.13564494934508925No Hit
CGCCAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCCTGTCTCT630.1335254970115722No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCC620.1314060446780552No Hit
CAGTAGACCTGGCGTCAGTAGACCTGGCGTCAGTAGACCTGTCTCTTATA620.1314060446780552No Hit
CCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGA620.1314060446780552No Hit
GGTCAAGCTGCTGCTGCAGGTCCAGCACGCCAGCAAGCAGATCGCCGCCG620.1314060446780552No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC610.12928659234453815No Hit
GTTTAAGACCAGCCGGACAACACAGCAAGATCCCGGCTCTAGCAAAACAT590.12504768767750413No Hit
CCAGGTTGATCTGGCGGTTGATGGCGGCCTCTGAGTCCTGGTGGTAGTTC590.12504768767750413No Hit
GCTCCGGACAGGTGTGGGGTGCACACTGAAAATGCCACGCTCCTTCCTGG590.12504768767750413No Hit
GGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCA580.12292823534398711No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCACTCTATCGCGTATGCC580.12292823534398711No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC550.11656987834343605No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA530.112330973676402No Hit
CTACTGACGCCAGGTCTACTGCTGTCTCTTATACACATCTCCGAGCCCAC530.112330973676402No Hit
GAGGACTACTCTGGATCAGGCTTCGGCTCCGGCTCCGGCTCTGGATCAGG530.112330973676402No Hit
GGTCGTCCATGATGGCGGTCTCTTTGGGGTCCAGCAGGTTGAACTCCCGA520.110211521342885No Hit
ATAGAGCTCTGAGTAGGCAGGTGGAGCATCGGTATAGGGTGGAGCCTGAG520.110211521342885No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA520.110211521342885No Hit
GGAGGGAAAAGTGGTAGATGCGAAGACCATCTTTACAGGGCATACGGCAG510.10809206900936799No Hit
TCCCTGGCCCTGCCCTTCGTCCTCATCCTCACTGTGCCGCTGCGGCGCGT510.10809206900936799No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA500.10597261667585095No Hit
GGTCTACTGACGCCAGGTCTACTGACGCCAGGTCCTGTCTCTTATACACA500.10597261667585095No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT500.10597261667585095No Hit
TAACTGCTCCAGGTTCGAGCTCGCCCTCTGCTCCTCACTGTCTCTTTCTG480.10173371200881691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)